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Antimicrobial Agents and Chemotherapy, March 2002, p. 925-928, Vol. 46, No. 3
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.3.925-928.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of a New Extended-Spectrum ß-Lactamase (TEM-87) Isolated in Proteus mirabilis during an Italian Survey

Dipartimento di Scienze e Tecnologie Biomediche,¹ Cattedra di Biochimica Clinica,³ Università di L'Aquila, L'Aquila, Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, Siena,⁴ Dipartimento Medico, Wyeth-Lederle Italia S.p.A., Aprilia, Italy²

Received 11 May 2001/ Returned for modification 23 September 2001/ Accepted 23 November 2001

A new natural TEM derivative, named TEM-87, was identified in a Proteus mirabilis isolate from an Italian hospital. Compared to TEM-1, TEM-87 contains the following mutations: E104K, R164C, and M182T. Kinetic analysis of TEM-87 revealed extended-spectrum activity against oxyimino cephalosporins (preferentially ceftazidime) and aztreonam. Expression of bla_TEM-87 in Escherichia coli decreased the host susceptibility to these drugs.

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