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Antimicrobial Agents and Chemotherapy, April 2002, p. 966-970, Vol. 46, No. 4
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.4.966-970.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular and Biochemical Characterization of Ambler Class A Extended-Spectrum ß-Lactamase CGA-1 from Chryseobacterium gleum

Samuel Bellais, Thierry Naas, and Patrice Nordmann*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre Cedex, France

Received 18 May 2001/ Returned for modification 23 September 2001/ Accepted 13 January 2002

Antibiotic susceptibility testing by disk diffusion of a Chryseobacterium gleum isolate, strain CIP 103039, showed a typical synergy image between clavulanic acid and expanded-spectrum cephalosporins. Shotgun cloning gave a recombinant plasmid in Escherichia coli that produced a ß-lactamase, CGA-1, with a pI value of 8.9 that conferred resistance to most penicillins (except ureidopenicillins) and narrow-spectrum cephalosporins and an intermediate susceptibility to expanded-spectrum cephalosporins and aztreonam. The CGA-1 amino acid sequence shared only 60% amino acid identity with CME-1 and CME-2 from Chryseobacterium meningosepticum, the most closely related ß-lactamases. CGA-1 was very likely chromosome encoded. It is a novel member of the PER subgroup of Ambler class A ß-lactamases (Bush functional group 2be).

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, April 2002, p. 966-970, Vol. 46, No. 4
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.4.966-970.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Girlich, D., Poirel, L., Schluter, A., Nordmann, P. (2005). TLA-2, a Novel Ambler Class A Expanded-Spectrum {beta}-Lactamase. Antimicrob. Agents Chemother. 49: 4767-4770 [Abstract] [Full Text]  
  • Kirby, J. T., Sader, H. S., Walsh, T. R., Jones, R. N. (2004). Antimicrobial Susceptibility and Epidemiology of a Worldwide Collection of Chryseobacterium spp.: Report from the SENTRY Antimicrobial Surveillance Program (1997-2001). J. Clin. Microbiol. 42: 445-448 [Abstract] [Full Text]  
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