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Antimicrobial Agents and Chemotherapy, May 2002, p. 1462-1468, Vol. 46, No. 5
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.5.1462-1468.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Plasmidic Extended-Spectrum ß-Lactamases in Vibrio cholerae O1 El Tor Isolates in Argentina{dagger}

Alejandro Petroni,1 Alejandra Corso,1 Roberto Melano,1 María Luisa Cacace,2 Ana María Bru,3 Alicia Rossi,1 and Marcelo Galas1*

Servicio Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán," Buenos Aires,1 Hospital San Vicente de Paul, Orán,,2 Hospital Juan D. Perón, Tartagal, Salta, Argentina3

Received 6 June 2001/ Returned for modification 29 September 2001/ Accepted 18 January 2002

Since 1992 there have been seven major outbreaks of cholera in Argentina. Susceptibility analysis of 1,947 isolates (40% of reported cases) of Vibrio cholerae O1 biotype El Tor suggested the presence of extended-spectrum ß-lactamases (ESBLs) in 28 isolates. Because of their different susceptibility profiles, V. cholerae isolates M1502, M1516, M1573, and M3030 (all of which are of the Ogawa serotype) were selected for the present study. By susceptibility analysis, isoelectric focusing, and PCR-based restriction fragment length polymorphism analysis, CTX-M-type enzymes were identified in three isolates, whereas a PER-2-type enzyme, in addition to a TEM-1-like enzyme, was identified in the other isolate. The presence of these ESBLs in V. cholerae isolates resulted in MICs well below those commonly observed for members of the family Enterobacteriaceae. Genes that encode both ESBLs were transferred to Escherichia coli by conjugation, together with all determinants of resistance to non-ß-lactam antibiotics (gentamicin, kanamycin, and sulfamethoxazole for all isolates; amikacin and streptomycin for three isolates; trimethoprim, tetracycline, and chloramphenicol for two isolates). Plasmid profile analysis and Southern blotting revealed the presence of single plasmids of about 150 kb in the four V. cholerae isolates and their respective transconjugants and revealed that the plasmids harbored genes encoding CTX-M-type or PER-2-type ESBLs. These results strongly suggest the broad spread of these ESBLs among genera belong to families other than the Enterobacteriaceae.

* Corresponding author. Mailing address: Servicio Antimicrobianos, INEI-ANLIS "Dr. Carlos G. Malbrán," Av. Velez Sarsfield 563 (1281), Buenos Aires, Argentina. Phone and Fax: 54-11-4303-2812. E-mail: mgalas{at}anlis.gov.ar.

{dagger} Dedicated to the memory of Alicia Rossi.

Antimicrobial Agents and Chemotherapy, May 2002, p. 1462-1468, Vol. 46, No. 5
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.5.1462-1468.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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