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Antimicrobial Agents and Chemotherapy, May 2002, p. 1535-1539, Vol. 46, No. 5
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.5.1535-1539.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Chromosomal System for Studying AmpC-Mediated ß-Lactam Resistance Mutation in Escherichia coli

Joseph F. Petrosino,1* Amanda R. Pendleton,1,{dagger} Joel H. Weiner,2 and Susan M. Rosenberg1,3,4*

Departments of Molecular and Human Genetics,1 Biochemistry and Molecular Biology,3 Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030-3411,4 Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7 Canada2

Received 5 September 2001/ Returned for modification 25 November 2001/ Accepted 9 February 2002

In some enterobacterial pathogens, but not in Escherichia coli, loss-of-function mutations in the ampD gene are a common route to ß-lactam antibiotic resistance. We constructed an assay system for studying mechanism(s) of enterobacterial ampD mutation using the well-developed genetics of E. coli. We integrated the Enterobacter ampRC genes into the E. coli chromosome. These cells acquire spontaneous recombination- and SOS response-independent ß-lactam resistance mutations in ampD. This chromosomal system is useful for studying mutation mechanisms that promote antibiotic resistance.

* Corresponding authors. Mailing address: Department of Molecular and Human Genetics, Baylor College of Medicine, Room S809A, Mail Stop BCM225, One Baylor Plaza, Houston, TX 77030-3411. Phone: (713) 798-6693. Fax: (713) 798-8967. E-mail for Joseph F. Petrosino: jp691032{at}bcm.tmc.edu. E-mail for Susan M. Rosenberg: smr{at}bcm.tmc.edu.

{dagger} Present address: Department of Cell Biology, Johns Hopkins School of Medicine, Baltimore, MD 21205.

Antimicrobial Agents and Chemotherapy, May 2002, p. 1535-1539, Vol. 46, No. 5
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.5.1535-1539.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Petrosino, J. F., Galhardo, R. S., Morales, L. D., Rosenberg, S. M. (2009). Stress-Induced {beta}-Lactam Antibiotic Resistance Mutation and Sequences of Stationary-Phase Mutations in the Escherichia coli Chromosome. J. Bacteriol. 191: 5881-5889 [Abstract] [Full Text]  
  • Kaneko, K., Okamoto, R., Nakano, R., Kawakami, S., Inoue, M. (2005). Gene Mutations Responsible for Overexpression of AmpC {beta}-Lactamase in Some Clinical Isolates of Enterobacter cloacae. J. Clin. Microbiol. 43: 2955-2958 [Abstract] [Full Text]  


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