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Antimicrobial Agents and Chemotherapy, May 2002, p. 1574-1576, Vol. 46, No. 5
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.5.1574-1576.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Macrolide and Tetracycline Resistance and Molecular Relationships of Clinical Strains of Streptococcus agalactiae

Esther Culebras,* Iciar Rodriguez-Avial, Carmen Betriu, Montserrat Redondo, and Juan J. Picazo

Department of Clinical Microbiology, Hospital Universitario San Carlos, 28040 Madrid, Spain

Received 8 October 2001/ Returned for modification 14 December 2001/ Accepted 24 January 2002

Mechanisms for tetracycline and macrolide resistance in 54 isolates of erythromycin-resistant Streptococcus agalactiae were analyzed by PCR. The erm(B), erm(A), and mef(A) genes, either alone or in combination, were detected in all the erythromycin-resistant isolates. The tet(M) and tet(O) genes were responsible for tetracycline resistance. Random amplification of polymorphic DNA indicated different clonal origins of the isolates.

* Corresponding author. Mailing address: Department of Clinical Microbiology, Hospital Universitario San Carlos, Martín Lagos s/n, 28040 Madrid, Spain. Phone: 34 913303269. Fax: 34 913303478. E-mail: eculebras{at}cbm.uam.es.

Antimicrobial Agents and Chemotherapy, May 2002, p. 1574-1576, Vol. 46, No. 5
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.5.1574-1576.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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