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Antimicrobial Agents and Chemotherapy, May 2002, p. 1602-1603, Vol. 46, No. 5
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.5.1602-1603.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Site-Specific Recombination with the Chromosomal tRNA^Leu Gene by the Large Conjugative Haemophilus Resistance Plasmid

Ioanna D. Dimopoulou,^, Joanne E. Russell,^, Zaini Mohd-Zain, Rebecca Herbert, and Derrick W. Crook^*

Interdepartmental Academic Unit of Infectious Diseases and Microbiology, Oxford University, John Radcliffe Hospital, Headington, Oxford OX3 9DU, United Kingdom

Received 25 October 2001/ Returned for modification 6 December 2001/ Accepted 4 February 2002

Characterization of the sequences involved in recombination of the Haemophilus plasmid p1056 with the Haemophilus influenzae chromosome produced evidence indicating site-specific recombination with chromosomal tRNA^Leu. attP sequences identical to those of p1056 were found in six plasmids of diverse origin, suggesting that a family of Haemophilus plasmids recombines with chromosomal tRNA^Leu.

Present address: Medical School of Alexandroupolis, University of Thrace, Alexandroupolis, Greece.

Present address: Laboratory for Physiology, Vrije Universiteit, 1081 BT Amsterdam, The Netherlands.

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