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Antimicrobial Agents and Chemotherapy, June 2002, p. 1674-1679, Vol. 46, No. 6
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.6.1674-1679.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Antimicrobial Activity of a Novel Catheter Lock Solution

Chirag B. Shah,1 Marc W. Mittelman,2 J. W. Costerton,3 Stephen Parenteau,4 Michael Pelak,2 Richard Arsenault,5 and Leonard A. Mermel4*

Biolink Corporation, Norwell, Massachusetts,1 Altran Corporation, Boston, Massachusetts,2 Montana State University, Bozeman, Montana,3 Division of Infectious Diseases, Rhode Island Hospital and Brown University School of Medicine, Providence, Rhode Island,4 Mycoscience, Inc., Willington, Connecticut5

Received 29 October 2001/ Returned for modification 4 January 2002/ Accepted 19 February 2002

Intravascular catheter-associated bloodstream infections significantly increase rates of morbidity and hospital costs. Microbial colonization and development of biofilms, which are known to be recalcitrant to antibiotic therapy, often lead to the loss of otherwise patent vascular access systems. We evaluated a new taurolidine- and citrate-based catheter lock solution (Neutrolin; Biolink Corporation, Norwell, Mass.) for its activity against planktonic microbes, antimicrobial activity in a catheter model, and biofilm eradication activity. In studies of planktonic microbes, after 24 h of contact, 675 mg of taurolidine-citrate solution per liter caused >99% reductions in the initial counts of Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Entercoccus faecalis. A solution of 13,500 mg/liter was cidal for Candida albicans. Ports and attached catheters inoculated with 50 to 600 CFU of these bloodstream isolates per ml were locked with heparin or the taurolidine-citrate solution. After 72 h, there was no growth in the taurolidine-citrate-treated devices but the heparin-treated devices exhibited growth in the range of 6 x 102 to 5 x 106 CFU/ml. Biofilms were developed on silicone disks in modified Robbins devices with broth containing 6% serum (initial counts, 106 to 108 CFU/cm2). The axenic biofilms were treated for 24 h with taurolidine-citrate or heparin. Taurolidine-citrate exposure resulted in a median reduction of 4.8 logs, whereas heparin treatment resulted in a median reduction of 1.7 logs (P < 0.01). No significant differences in the effects of the two treatments against P. aeruginosa and C. albicans were observed. These findings suggest that taurolidine-citrate is a promising combination agent for the prevention and treatment of intravascular catheter-related infections.

* Corresponding author. Mailing address: Division of Infectious Diseases, Rhode Island Hospital, 593 Eddy St., Providence, RI 02903. Phone: (401) 444-8130. Fax: (401) 444-8154. E-mail: lmermel{at}lifespan.org.

Antimicrobial Agents and Chemotherapy, June 2002, p. 1674-1679, Vol. 46, No. 6
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.6.1674-1679.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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