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Antimicrobial Agents and Chemotherapy, June 2002, p. 1980-1984, Vol. 46, No. 6
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.6.1980-1984.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Effect of Ciprofloxacin on Killing of Actinobacillus actinomycetemcomitans by Polymorphonuclear Leukocytes

David A. Cacchillo1 and John D. Walters1,2*

Section of Periodontology, College of Dentistry,1 Department of Molecular and Cellular Biochemistry, College of Medicine and Public Health, The Ohio State University Health Sciences Center, Columbus, Ohio2

Received 29 June 2001/ Returned for modification 18 November 2001/ Accepted 6 February 2002

Actinobacillus actinomycetemcomitans, a pathogen associated with aggressive periodontitis, resists phagocytic killing by polymorphonuclear leukocytes (PMNs). It is susceptible to ciprofloxacin, which PMNs actively accumulate. This study tested the hypothesis that ciprofloxacin-loaded PMNs are more effective at killing A. actinomycetemcomitans than control PMNs. Isolated human PMNs were loaded by brief incubation with 0.5 µg of ciprofloxacin/ml. Opsonized bacteria (ATCC 43718) were incubated at 37°C with control and ciprofloxacin-loaded PMNs and in the presence and absence of 0.5 µg of ciprofloxacin/ml. When assayed at bacteria-to-PMN ratios of 30:1 and 90:1, ciprofloxacin-loaded PMNs killed significantly more bacteria and achieved significantly shorter half times for killing than control PMNs (P < 0.05; Tukey's test). At ratios of 3:1 and 10:1, these differences were not significant.

* Corresponding author. Mailing address: John D. Walters College of Dentistry, The Ohio State University, 305 West 12th Ave., P.O. Box 182357, Columbus, OH 43218-2357. Phone: (614) 292-1169. Fax: (614) 292-2438. E-mail: walters.2{at}osu.edu.

Antimicrobial Agents and Chemotherapy, June 2002, p. 1980-1984, Vol. 46, No. 6
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.6.1980-1984.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • TOZUM, T. F., YILDIRIM, A., CAGLAYAN, F., DINCEL, A., BOZKURT, A. (2004). Serum and gingival crevicular fluid levels of ciprofloxacin in patients with periodontitis. Journal of the American Dental Association 135: 1728-1732 [Abstract] [Full Text]  
  • Walters, J. D., Nakkula, R. J. (2003). Ciprofloxacin Transport by Chemoattractant-Activated Polymorphonuclear Leukocytes: Regulation by Priming and Protein Kinase C. Antimicrob. Agents Chemother. 47: 3345-3348 [Abstract] [Full Text]  


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