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Antimicrobial Agents and Chemotherapy, June 2002, p. 1985-1988, Vol. 46, No. 6
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.6.1985-1988.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

CTX-M-14, a Plasmid-Mediated CTX-M Type Extended-Spectrum ß-Lactamase Isolated from Escherichia coli

L. Ma,1 Yoshikazu Ishii,2 Feng-Yee Chang,3 Keizo Yamaguchi,2 Monto Ho,1 and L. K. Siu1*

Division of Clinical Research, National Health Research Institutes,1 Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan,3 Department of Microbiology, Toho University School of Medicine, Tokyo, Japan2

Received 25 July 2001/ Returned for modification 23 November 2001/ Accepted 16 March 2002

Four Escherichia coli isolates harboring CTX-M-14, with a single Ala231->Val substitution compared to CTX-M-9, had three different ribotypes. Cefotaxime resistance was plasmid encoded and conjugatively transferable. Three isolates had the same plasmid restriction enzyme digestion profile, suggesting clonal spread of a resistant plasmid. A high kcat/Km value for cefotaxime (20.3 µM-1 s-1) but low values for ceftazidime and aztreonam (<0.02 µM-1 s-1) were observed in hydrolysis assays, indicating resistance to cefotaxime (MIC >= 64 µg/ml) but susceptibility to ceftazidime (MIC <= 2 µg/ml).

* Corresponding author. Mailing address: Division of Clinical Research, National Health Research Institutes (99), 128 Yen-Chiu-Yuan Rd., Sec. 2, Taipei 11529, Taiwan. Phone: 886 2 26524094. Fax: 886 2 27890254. E-mail: lksiu{at}nhri.org.tw.

Antimicrobial Agents and Chemotherapy, June 2002, p. 1985-1988, Vol. 46, No. 6
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.6.1985-1988.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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