This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ma, L. Articles by Siu, L. K. Search for Related Content PubMed PubMed Citation Articles by Ma, L. Articles by Siu, L. K.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, June 2002, p. 1985-1988, Vol. 46, No. 6
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.6.1985-1988.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

CTX-M-14, a Plasmid-Mediated CTX-M Type Extended-Spectrum ß-Lactamase Isolated from Escherichia coli

Division of Clinical Research, National Health Research Institutes,¹ Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan,³ Department of Microbiology, Toho University School of Medicine, Tokyo, Japan²

Received 25 July 2001/ Returned for modification 23 November 2001/ Accepted 16 March 2002

Four Escherichia coli isolates harboring CTX-M-14, with a single Ala231Val substitution compared to CTX-M-9, had three different ribotypes. Cefotaxime resistance was plasmid encoded and conjugatively transferable. Three isolates had the same plasmid restriction enzyme digestion profile, suggesting clonal spread of a resistant plasmid. A high k_cat/K_m value for cefotaxime (20.3 µM^-1 s^-1) but low values for ceftazidime and aztreonam (<0.02 µM^-1 s^-1) were observed in hydrolysis assays, indicating resistance to cefotaxime (MIC 64 µg/ml) but susceptibility to ceftazidime (MIC 2 µg/ml).

This article has been cited by other articles:

• Lavigne, J.-P., Marchandin, H., Delmas, J., Moreau, J., Bouziges, N., Lecaillon, E., Cavalie, L., Jean-Pierre, H., Bonnet, R., Sotto, A. (2007). CTX-M {beta}-Lactamase-Producing Escherichia coli in French Hospitals: Prevalence, Molecular Epidemiology, and Risk Factors. J. Clin. Microbiol. 45: 620-626 [Abstract] [Full Text]  
• Paterson, D. L., Bonomo, R. A. (2005). Extended-Spectrum {beta}-Lactamases: a Clinical Update. Clin. Microbiol. Rev. 18: 657-686 [Abstract] [Full Text]  
• Chia, J.-H., Chu, C., Su, L.-H., Chiu, C.-H., Kuo, A.-J., Sun, C.-F., Wu, T.-L. (2005). Development of a Multiplex PCR and SHV Melting-Curve Mutation Detection System for Detection of Some SHV and CTX-M {beta}-Lactamases of Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae in Taiwan. J. Clin. Microbiol. 43: 4486-4491 [Abstract] [Full Text]  
• Li, H., Li, J. B. (2005). Detection of Five Novel CTX-M-Type Extended Spectrum Beta-Lactamases with One to Three CTX-M-14 Point Mutations in Isolates from Hefei, Anhui Province, China. J. Clin. Microbiol. 43: 4301-4302 [Full Text]  
• Pitout, J. D. D., Gregson, D. B., Church, D. L., Elsayed, S., Laupland, K. B. (2005). Community-Wide Outbreaks of Clonally Related CTX-M-14 {beta}-Lactamase-Producing Escherichia coli Strains in the Calgary Health Region. J. Clin. Microbiol. 43: 2844-2849 [Abstract] [Full Text]  
• Pitout, J. D. D., Hossain, A., Hanson, N. D. (2004). Phenotypic and Molecular Detection of CTX-M-{beta}-Lactamases Produced by Escherichia coli and Klebsiella spp.. J. Clin. Microbiol. 42: 5715-5721 [Abstract] [Full Text]  
• Kimura, S., Ishiguro, M., Ishii, Y., Alba, J., Yamaguchi, K. (2004). Role of a Mutation at Position 167 of CTX-M-19 in Ceftazidime Hydrolysis. Antimicrob. Agents Chemother. 48: 1454-1460 [Abstract] [Full Text]  
• Bonnet, R. (2004). Growing Group of Extended-Spectrum {beta}-Lactamases: the CTX-M Enzymes. Antimicrob. Agents Chemother. 48: 1-14 [Full Text]  
• Edelstein, M., Pimkin, M., Palagin, I., Edelstein, I., Stratchounski, L. (2003). Prevalence and Molecular Epidemiology of CTX-M Extended-Spectrum {beta}-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Russian Hospitals. Antimicrob. Agents Chemother. 47: 3724-3732 [Abstract] [Full Text]  
• Brinas, L., Moreno, M. A., Zarazaga, M., Porrero, C., Saenz, Y., Garcia, M., Dominguez, L., Torres, C. (2003). Detection of CMY-2, CTX-M-14, and SHV-12 {beta}-Lactamases in Escherichia coli Fecal-Sample Isolates from Healthy Chickens. Antimicrob. Agents Chemother. 47: 2056-2058 [Abstract] [Full Text]