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Antimicrobial Agents and Chemotherapy, June 2002, p. 2024-2028, Vol. 46, No. 6
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.6.2024-2028.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Cloning of a Cation Efflux Pump Gene Associated with Chlorhexidine Resistance in Klebsiella pneumoniae

Chi-Tai Fang,1 Haur-Chuan Chen,2 Yi-Ping Chuang,2 Shan-Chwen Chang,1 and Jin-Town Wang1,2*

Department of Internal Medicine, National Taiwan University Hospital,1 Department of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan2

Received 14 August 2001/ Returned for modification 3 October 2001/ Accepted 7 March 2002

Expression libraries of a chlorhexidine-resistant Klebsiella pneumoniae strain were constructed and transformed into Escherichia coli XLOLR. Twenty chlorhexidine-resistant transformants were obtained after selection. All clones contained a novel 903-nucleotide locus. Its sequences were compatible with a cation efflux pump, and the locus was thus designated as cepA. Retransformation using cepA-containing plasmids conferred chlorhexidine resistance to both XLOLR and a chlorhexidine-sensitive K. pneumoniae strain. Therefore, CepA is associated with chlorhexidine resistance and may act as a cation efflux pump.

* Corresponding author. Mailing address: Department of Microbiology, National Taiwan University College of Medicine, 1, Jen-Ai Rd., Taipei 100, Taiwan. Phone: 886-2-23123456, ext. 8292. Fax: 886-2-23948718. E-mail: wangjt{at}ccms.ntu.edu.tw.

Antimicrobial Agents and Chemotherapy, June 2002, p. 2024-2028, Vol. 46, No. 6
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.6.2024-2028.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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  • Chou, H.-C., Lee, C.-Z., Ma, L.-C., Fang, C.-T., Chang, S.-C., Wang, J.-T. (2004). Isolation of a Chromosomal Region of Klebsiella pneumoniae Associated with Allantoin Metabolism and Liver Infection. Infect. Immun. 72: 3783-3792 [Abstract] [Full Text]  
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