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Antimicrobial Agents and Chemotherapy, June 2002, p. 2024-2028, Vol. 46, No. 6
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.6.2024-2028.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Cloning of a Cation Efflux Pump Gene Associated with Chlorhexidine Resistance in Klebsiella pneumoniae

Department of Internal Medicine, National Taiwan University Hospital,¹ Department of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan²

Received 14 August 2001/ Returned for modification 3 October 2001/ Accepted 7 March 2002

Expression libraries of a chlorhexidine-resistant Klebsiella pneumoniae strain were constructed and transformed into Escherichia coli XLOLR. Twenty chlorhexidine-resistant transformants were obtained after selection. All clones contained a novel 903-nucleotide locus. Its sequences were compatible with a cation efflux pump, and the locus was thus designated as cepA. Retransformation using cepA-containing plasmids conferred chlorhexidine resistance to both XLOLR and a chlorhexidine-sensitive K. pneumoniae strain. Therefore, CepA is associated with chlorhexidine resistance and may act as a cation efflux pump.

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