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Antimicrobial Agents and Chemotherapy, June 2002, p. 2049-2051, Vol. 46, No. 6
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.6.2049-2051.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Effects of Chemical and Physical Agents on Viability and Infectivity of Encephalitozoon intestinalis Determined by Cell Culture and Flow Cytometry

Maud Santillana-Hayat,1 Claudine Sarfati,1 Sandra Fournier,2 Françoise Chau,3 Raphaël Porcher,4 Jean-Michel Molina,2 and Francis Derouin1*

Parasitology-Mycology Laboratory,1 Department of Infectious Diseases,2 Department of Biostatistics, Saint-Louis Hospital, AP-HP and EA 3520, University of Paris 7,4 INSERM, Bichat Hospital, Paris, France3

Received 10 October 2001/ Returned for modification 27 November 2001/ Accepted 4 March 2002

We combined tissue culture and flow cytometry to assess the activities of various temperatures, chemicals, and disinfectants on the viability and infectivity of spores of Encephalitozoon intestinalis. Surfanios and benzalkonium chloride, disinfectants currently used in the hospital, were remarkably efficient in destroying spore viability and infectivity.

* Corresponding author. Mailing address: Laboratoire de Parasitologie-Mycologie, Saint-Louis Hospital, AP-HP, 1, Avenue Claude Vellefaux, 75475, Paris Cedex 10, France. Phone: 33 1 42 49 95 01. Fax: 33 1 42 49 48 03. E-mail: paracord{at}wanadoo.fr.

Antimicrobial Agents and Chemotherapy, June 2002, p. 2049-2051, Vol. 46, No. 6
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.6.2049-2051.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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