This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An author's correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Svensson, M.
Right arrow Articles by Nilsson, L. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Svensson, M.
Right arrow Articles by Nilsson, L. E.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, July 2002, p. 2244-2248, Vol. 46, No. 7
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.7.2244-2248.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Pharmacodynamic Effects of Nitroimidazoles Alone and in Combination with Clarithromycin on Helicobacter pylori

Margareta Svensson,1,2 Magnus Ström,2 Maud Nilsson,1 Mikael Sörberg,3 and Lennart E. Nilsson1*

Divisions of Clinical Microbiology,1 Gastroenterology, Department of Molecular and Clinical Medicine, Faculty of Health Sciences, SE-581 85 Linköping,2 Infectious Diseases Unit, Department of Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden3

Received 15 December 2000/ Returned for modification 2 June 2001/ Accepted 29 March 2002

Pharmacodynamic studies of Helicobacter pylori exposed to metronidazole and tinidazole alone and in combination with clarithromycin were performed by bioluminescence assay of intracellular ATP. The pharmacodynamic parameter control-related effective regrowth time (CERT) was used. CERT is defined as the time required for the resumption of logarithmic growth and a return of the level of growth to the preexposure inoculum in the test culture minus the corresponding time in the control culture. CERT measures the combined effects of the initial level of killing and postantibiotic effect. The incubation times and drug concentrations were chosen according to their half-lives and their clinically achievable concentrations. The study shows that the parameter CERT is useful for the testing of antibiotic combinations. The CERTs induced by clarithromycin, metronidazole, and tinidazole alone and in the combinations tested were concentration dependent, with no maximum response, indicating that the use of high doses may be preferable. The combinations with the highest concentrations induced synergistic effects and prevented regrowth. The use of tinidazole in combination with clarithromycin proved to give the longest CERTs, indicating that this is the most effective combination.

* Corresponding author. Mailing address: Division of Clinical Microbiology, Department of Molecular and Clinical Medicine, Faculty of Health Sciences, SE-581 85 Linköping, Sweden. Phone: 46 13 222098. Fax: 46 13 224596. E-mail: len{at}imk.liu.se.

Antimicrobial Agents and Chemotherapy, July 2002, p. 2244-2248, Vol. 46, No. 7
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.7.2244-2248.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»