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Antimicrobial Agents and Chemotherapy, July 2002, p. 2269-2272, Vol. 46, No. 7
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.7.2269-2272.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Fluorescence Assay for Studying the Ability of Macrolides To Induce Production of Ribosomal Methylase

Gervais Clarebout and Roland Leclercq*

Service de Microbiologie, UPRES EA 2128, Hôpital Côte de Nacre, Université de Caen, 14033 Caen Cedex, France

Received 27 September 2001/ Returned for modification 5 January 2002/ Accepted 7 April 2002

A screening assay to test the inducing capacity of macrolides by fusing the attenuator of the inducible erm(B) gene from Streptococcus pneumoniae HM28 with the gfpmut1 gene has been designed. Fluorescence was detected under UV light around disks impregnated with inducer macrolides (erythromycin or azithromycin) but not with noninducer ketolides. Induction could be quantified by fluorometry.

* Corresponding author. Mailing address: CHU de Caen, Service de Microbiologie, Avenue Côte de Nacre, 14033 Caen Cedex, France. Phone: (33) 02 31 06 45 72. Fax: (33) 02 31 06 45 73. E-mail: leclercq-r{at}chu-caen.fr.

Antimicrobial Agents and Chemotherapy, July 2002, p. 2269-2272, Vol. 46, No. 7
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.7.2269-2272.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bailey, M., Chettiath, T., Mankin, A. S. (2008). Induction of erm(C) Expression by Noninducing Antibiotics. Antimicrob. Agents Chemother. 52: 866-874 [Abstract] [Full Text]  
  • Depardieu, F., Podglajen, I., Leclercq, R., Collatz, E., Courvalin, P. (2007). Modes and Modulations of Antibiotic Resistance Gene Expression. Clin. Microbiol. Rev. 20: 79-114 [Abstract] [Full Text]  


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