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Antimicrobial Agents and Chemotherapy, August 2002, p. 2400-2408, Vol. 46, No. 8
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.8.2400-2408.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Class 1 Integron Containing a New Gene Cassette, aadA10, Associated with Tn1404 from R151

Sally R. Partridge,1,2 Christina M. Collis,1 and Ruth M. Hall1*

CSIRO Molecular Science, North Ryde, NSW 2113,1 Department of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia2

Received 19 November 2001/ Returned for modification 12 March 2002/ Accepted 23 April 2002

The carbenicillin, gentamicin, kanamycin, streptomycin, spectinomycin, sulfonamide, and tobramycin resistance determinants found on Pseudomonas aeruginosa plasmid R151 have previously been shown to translocate to another plasmid, R388, and it was inferred that a transposon, Tn1404, carried the resistance determinants. Sequencing of the cassette array from the plasmid known as R388::Tn1404 revealed two known gene cassettes, oxa10 and aadB, and a previously unidentified cassette determining resistance to streptomycin and spectinomycin, here designated aadA10, in the order oxa10-aadB-aadA10. These cassettes replaced the dfrB2-orfA cassette array of R388, indicating that movement of the resistance determinants from R151 to R388 resulted from recombinational exchange between two class 1 integrons rather than transposition. The AadA10 protein is most closely related to AadA6 (85% identical) and AadA7 (80% identical). The aadA10 cassette found here has only a simple site containing a 7-bp spacer derived from attI1 in place of a 59-base element and is likely to represent a derivative of the complete cassette. IntI1-mediated deletion of the aadA10 cassette was not detected, indicating that this single simple site is either inactive or only weakly active.

* Corresponding author: Mailing address: CSIRO Molecular Science, Sydney Laboratory, P.O. Box 184, North Ryde, NSW 1670, Australia. Phone: 61-2-9490 5162. Fax: 61-2-9490 5005. E-mail: ruth.hall{at}csiro.au.

Antimicrobial Agents and Chemotherapy, August 2002, p. 2400-2408, Vol. 46, No. 8
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.8.2400-2408.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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