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Antimicrobial Agents and Chemotherapy, August 2002, p. 2644-2647, Vol. 46, No. 8
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.8.2644-2647.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

gyrA Polymorphism in Campylobacter jejuni: Detection of gyrA Mutations in 162 C. jejuni Isolates by Single-Strand Conformation Polymorphism and DNA Sequencing

Antti Hakanen,1,2* Jari Jalava,1 Pirkko Kotilainen,2 Hannele Jousimies-Somer,3,4 Anja Siitonen,5 and Pentti Huovinen1

Antimicrobial Research Laboratory, National Public Health Institute,1 Department of Medicine, Turku University Central Hospital, Turku,2 Anaerobe Reference Laboratory,3 Laboratory of Enteric Pathogens, National Public Health Institute,5 Microbiology Laboratory, The Mehiläinen Hospital, Helsinki, Finland4

Received 15 November 2001/ Returned for modification 12 February 2002/ Accepted 26 April 2002

Mutations in the quinolone resistance-determining region of the gyrA gene from 138 ciprofloxacin-resistant (MIC, >=4 µg/ml) and 24 ciprofloxacin-susceptible (MIC, <=1 µg/ml) clinical Campylobacter jejuni isolates were subjected to single-strand conformation polymorphism analysis and sequencing. All of the isolates could be assigned to three genotypic clusters based on silent mutations. All resistant isolates had a point mutation at codon 86.

* Corresponding author. Mailing address: Antimicrobial Research Laboratory, National Public Health Institute, P.O. Box 57, 20521 Turku, Finland. Phone: 358 2 2519255. Fax: 358 2 2519254. E-mail: antti.hakanen{at}utu.fi.

Antimicrobial Agents and Chemotherapy, August 2002, p. 2644-2647, Vol. 46, No. 8
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.8.2644-2647.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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