This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thungapathra, M.
Right arrow Articles by Ghosh, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thungapathra, M.
Right arrow Articles by Ghosh, A.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, September 2002, p. 2948-2955, Vol. 46, No. 9
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.9.2948-2955.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Occurrence of Antibiotic Resistance Gene Cassettes aac(6')-Ib, dfrA5, dfrA12, and ereA2 in Class I Integrons in Non-O1, Non-O139 Vibrio cholerae Strains in India

M. Thungapathra,1 Amita,1 Kislay K. Sinha,1 Saumya Ray Chaudhuri,1 Pallavi Garg,2 Thandavarayan Ramamurthy,2 Gopinath Balakrish Nair,2,3 and Amit Ghosh1*

Institute of Microbial Technology, Chandigarh,1 National Institute of Cholera and Enteric Diseases, Calcutta, India,2 International Centre for Diarrhoeal Disease Research, Dhaka 1000, Bangladesh3

Received 11 December 2001/ Returned for modification 10 April 2002/ Accepted 31 May 2002

Molecular mechanisms of multidrug resistance in Vibrio cholerae belonging to non-O1, non-O139 serogroups isolated during 1997 to 1998 in Calcutta, India, were investigated. Out of the 94 strains examined, 22 strains were found to have class I integrons. The gene cassettes identified were dfrA1, dfrA15, dfrA5, and dfrA12 for trimethoprim; aac(6')-Ib for amikacin and tobramycin; aadA1 and aadA2 for streptomycin and spectinomycin; and ereA2 for erythromycin resistance. To our knowledge, this is the first report of the presence of dfrA5, dfrA12, aac(6')-Ib, and ereA2 cassettes in class I integrons of V. cholerae. Forty-three of 94 strains also had plasmids, and out of these, 14 contained both class I integrons and plasmids. Pulsed-field gel electrophoresis followed by Southern hybridization revealed that in the 14 plasmid-bearing strains, class I integrons resided either on chromosomes, on plasmids, or on both. Our results indicated that besides class I integrons and plasmids, a conjugative transposon element, SXT, possibly contributed to the multiple antibiotic resistance.

* Corresponding author. Mailing address: Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India. Phone: 91-172-690785/690684. Fax: 91-172-690585/690632. E-mail: director{at}imtech.res.in.

Antimicrobial Agents and Chemotherapy, September 2002, p. 2948-2955, Vol. 46, No. 9
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.9.2948-2955.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kumar, P., Thomas, S. (2009). Presence of dfr6 Gene Cassette in Superintegron of Non-O1/Non-O139 Strain of Vibrio cholerae. Antimicrob. Agents Chemother. 53: 4959-4960 [Full Text]  
  • Talyzina, N. M., Ingvarsson, P. K., Zhu, J., Wai, S. N., Andersson, A. (2009). Molecular Diversification in the Quorum-Sensing System of Vibrio cholerae: Role of Natural Selection in the Emergence of Pandemic Strains. Appl. Environ. Microbiol. 75: 3808-3812 [Abstract] [Full Text]  
  • Khan, A. A., Ponce, E., Nawaz, M. S., Cheng, C.-M., Khan, J. A., West, C. S. (2009). Identification and Characterization of Class 1 Integron Resistance Gene Cassettes among Salmonella Strains Isolated from Imported Seafood. Appl. Environ. Microbiol. 75: 1192-1196 [Abstract] [Full Text]  
  • Dongre, M., Tripathi, R., Jain, V., Raychaudhuri, S. (2008). Functional independence of a variant LuxOPL91 from a non-O1 non-O139 Vibrio cholerae over the activity of CsrA and Fis. J Med Microbiol 57: 1041-1045 [Full Text]  
  • Ramachandran, D., Bhanumathi, R., Singh, D. V. (2007). Multiplex PCR for detection of antibiotic resistance genes and the SXT element: application in the characterization of Vibrio cholerae. J Med Microbiol 56: 346-351 [Abstract] [Full Text]  
  • Khan, A. A., Cheng, C.-M., Van, K. T., West, C. S., Nawaz, M. S., Khan, S. A. (2006). Characterization of class 1 integron resistance gene cassettes in Salmonella enterica serovars Oslo and Bareily from imported seafood. J Antimicrob Chemother 58: 1308-1310 [Full Text]  
  • Agerso, Y., Peirano, G., Aarestrup, F. M. (2006). dfrA25, a novel trimethoprim resistance gene from Salmonella Agona isolated from a human urine sample in Brazil. J Antimicrob Chemother 58: 1044-1047 [Abstract] [Full Text]  
  • Srinivasan, V. B., Virk, R. K., Kaundal, A., Chakraborty, R., Datta, B., Ramamurthy, T., Mukhopadhyay, A. K., Ghosh, A. (2006). Mechanism of Drug Resistance in Clonally Related Clinical Isolates of Vibrio fluvialis Isolated in Kolkata, India. Antimicrob. Agents Chemother. 50: 2428-2432 [Abstract] [Full Text]  
  • Ceccarelli, D., Salvia, A. M., Sami, J., Cappuccinelli, P., Colombo, M. M. (2006). New Cluster of Plasmid-Located Class 1 Integrons in Vibrio cholerae O1 and a dfrA15 Cassette-Containing Integron in Vibrio parahaemolyticus Isolated in Angola. Antimicrob. Agents Chemother. 50: 2493-2499 [Abstract] [Full Text]  
  • Shi, L., Fujihara, K., Sato, T., Ito, H., Garg, P., Chakrabarty, R., Ramamurthy, T., Nair, G. B., Takeda, Y., Yamasaki, S. (2006). Distribution and characterization of integrons in various serogroups of Vibrio cholerae strains isolated from diarrhoeal patients between 1992 and 2000 in Kolkata, India.. J Med Microbiol 55: 575-583 [Abstract] [Full Text]  
  • Ahmed, A. M., Kawaguchi, F., Shimamoto, T. (2006). Class 2 integrons in Vibrio cholerae.. J Med Microbiol 55: 643-644 [Full Text]  
  • Vora, G. J., Meador, C. E., Bird, M. M., Bopp, C. A., Andreadis, J. D., Stenger, D. A. (2005). Microarray-based detection of genetic heterogeneity, antimicrobial resistance, and the viable but nonculturable state in human pathogenic Vibrio spp.. Proc. Natl. Acad. Sci. USA 102: 19109-19114 [Abstract] [Full Text]  
  • Levings, R. S., Lightfoot, D., Partridge, S. R., Hall, R. M., Djordjevic, S. P. (2005). The Genomic Island SGI1, Containing the Multiple Antibiotic Resistance Region of Salmonella enterica Serovar Typhimurium DT104 or Variants of It, Is Widely Distributed in Other S. enterica Serovars. J. Bacteriol. 187: 4401-4409 [Abstract] [Full Text]  
  • Petroni, A., Melano, R. G., Saka, H. A., Garutti, A., Mange, L., Pasteran, F., Rapoport, M., Miranda, M., Faccone, D., Rossi, A., Hoffman, P. S., Galas, M. F. (2004). CARB-9, a Carbenicillinase Encoded in the VCR Region of Vibrio cholerae Non-O1, Non-O139 Belongs to a Family of Cassette-Encoded {beta}-Lactamases. Antimicrob. Agents Chemother. 48: 4042-4046 [Abstract] [Full Text]  
  • Ojo, K. K., Ulep, C., Van Kirk, N., Luis, H., Bernardo, M., Leitao, J., Roberts, M. C. (2004). The mef(A) Gene Predominates among Seven Macrolide Resistance Genes Identified in Gram-Negative Strains Representing 13 Genera, Isolated from Healthy Portuguese Children. Antimicrob. Agents Chemother. 48: 3451-3456 [Abstract] [Full Text]  
  • Iwanaga, M., Toma, C., Miyazato, T., Insisiengmay, S., Nakasone, N., Ehara, M. (2004). Antibiotic Resistance Conferred by a Class I Integron and SXT Constin in Vibrio cholerae O1 Strains Isolated in Laos. Antimicrob. Agents Chemother. 48: 2364-2369 [Abstract] [Full Text]  
  • Ahmed, A. M., Nakagawa, T., Arakawa, E., Ramamurthy, T., Shinoda, S., Shimamoto, T. (2004). New aminoglycoside acetyltransferase gene, aac(3)-Id, in a class 1 integron from a multiresistant strain of Vibrio fluvialis isolated from an infant aged 6 months. J Antimicrob Chemother 53: 947-951 [Abstract] [Full Text]  
  • Burrus, V., Waldor, M. K. (2004). Formation of SXT Tandem Arrays and SXT-R391 Hybrids. J. Bacteriol. 186: 2636-2645 [Abstract] [Full Text]  
  • Sarno, R., Ha, H., Weinsetel, N., Tolmasky, M. E. (2003). Inhibition of Aminoglycoside 6'-N-Acetyltransferase Type Ib-Mediated Amikacin Resistance by Antisense Oligodeoxynucleotides. Antimicrob. Agents Chemother. 47: 3296-3304 [Abstract] [Full Text]  
  • Biskri, L., Mazel, D. (2003). Erythromycin Esterase Gene ere(A) Is Located in a Functional Gene Cassette in an Unusual Class 2 Integron. Antimicrob. Agents Chemother. 47: 3326-3331 [Abstract] [Full Text]  
  • Vakulenko, S. B., Mobashery, S. (2003). Versatility of Aminoglycosides and Prospects for Their Future. Clin. Microbiol. Rev. 16: 430-450 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»