This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Braibant, M.
Right arrow Articles by Zygmunt, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Braibant, M.
Right arrow Articles by Zygmunt, M. S.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, September 2002, p. 3050-3053, Vol. 46, No. 9
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.9.3050-3053.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Functional Characterization of Brucella melitensis NorMI, an Efflux Pump Belonging to the Multidrug and Toxic Compound Extrusion Family

Martine Braibant,,{dagger} Laurence Guilloteau,* and Michel S. Zygmunt

Unité de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France

Received 23 January 2002/ Returned for modification 11 April 2002/ Accepted 3 June 2002

Two putative proteins (NorMI and NorMII) similar to the multidrug efflux protein NorM of Vibrio parahaemolyticus are encoded by the Brucella melitensis 16 M genome. We show that a drug-hypersusceptible Escherichia coli strain overexpressing NorMI displays increased resistance to norfloxacin, ciprofloxacin, gentamicin, tetraphenylphosphonium ion, acriflavine, and berberine. This elevated resistance was proven to be mediated by an energy-dependent efflux mechanism. NorMI belongs to the multidrug and toxic compound extrusion family and is the first multidrug efflux protein identified in Brucella spp.

* Corresponding author. Mailing address: Unité de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France. Phone: 33-2-47 42 78 90. Fax: 33-2-47 42 77 79. E-mail: Laurence.Guilloteau{at}tours.inra.fr.

{dagger} Present address: Station de Pathologie Aviaire et Parasitologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France.

Antimicrobial Agents and Chemotherapy, September 2002, p. 3050-3053, Vol. 46, No. 9
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.9.3050-3053.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Posadas, D. M., Martin, F. A., Sabio y Garcia, J. V., Spera, J. M., Delpino, M. V., Baldi, P., Campos, E., Cravero, S. L., Zorreguieta, A. (2007). The TolC Homologue of Brucella suis Is Involved in Resistance to Antimicrobial Compounds and Virulence. Infect. Immun. 75: 379-389 [Abstract] [Full Text]  
  • Singh, A. K., Haldar, R., Mandal, D., Kundu, M. (2006). Analysis of the Topology of Vibrio cholerae NorM and Identification of Amino Acid Residues Involved in Norfloxacin Resistance. Antimicrob. Agents Chemother. 50: 3717-3723 [Abstract] [Full Text]  
  • Grillo, M. J., De Miguel, M. J., Munoz, P. M., Marin, C. M., Ariza, J., Blasco, J. M. (2006). Efficacy of several antibiotic combinations against Brucella melitensis Rev 1 experimental infection in BALB/c mice. J Antimicrob Chemother 58: 622-626 [Abstract] [Full Text]  
  • Piddock, L. J. V. (2006). Clinically Relevant Chromosomally Encoded Multidrug Resistance Efflux Pumps in Bacteria. Clin. Microbiol. Rev. 19: 382-402 [Abstract] [Full Text]  
  • Tauch, A., Kaiser, O., Hain, T., Goesmann, A., Weisshaar, B., Albersmeier, A., Bekel, T., Bischoff, N., Brune, I., Chakraborty, T., Kalinowski, J., Meyer, F., Rupp, O., Schneiker, S., Viehoever, P., Puhler, A. (2005). Complete Genome Sequence and Analysis of the Multiresistant Nosocomial Pathogen Corynebacterium jeikeium K411, a Lipid-Requiring Bacterium of the Human Skin Flora. J. Bacteriol. 187: 4671-4682 [Abstract] [Full Text]  
  • Otsuka, M., Yasuda, M., Morita, Y., Otsuka, C., Tsuchiya, T., Omote, H., Moriyama, Y. (2005). Identification of Essential Amino Acid Residues of the NorM Na+/Multidrug Antiporter in Vibrio parahaemolyticus. J. Bacteriol. 187: 1552-1558 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»