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Antimicrobial Agents and Chemotherapy, September 2002, p. 3084-3087, Vol. 46, No. 9
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.9.3084-3087.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Comparative Evaluation of Disk Diffusion with Microdilution Assay in Susceptibility Testing of Caspofungin against Aspergillus and Fusarium Isolates

Sevtap Arikan,* Victor Paetznick, and John H. Rex

Division of Infectious Diseases, Department of Internal Medicine, Center for the Study of Emerging and Reemerging Pathogens, University of Texas Medical School, Houston, Texas 77030

Received 7 December 2001/ Returned for modification 25 March 2002/ Accepted 5 June 2002

We compared the disk diffusion and broth microdilution methods for susceptibility testing of caspofungin against Aspergillus (n = 78) and Fusarium (n = 22) isolates. Microdilution testing followed the NCCLS M-38P guidelines but was performed in antibiotic medium 3 supplemented to 2% glucose (AM3). Disk diffusion assays were performed on AM3 agar plates with a 2-µg caspofungin disk. By both methods, caspofungin showed favorable activity against Aspergillus isolates and no activity against Fusarium isolates. In the disk-based format, intrazonal growth that was not influenced by the drug concentration gradient was consistently observed for all of the Aspergillus isolates tested.

* Corresponding author. Present address: Department of Microbiology and Clinical Microbiology, Hacettepe University Medical School, 06100 Ankara, Turkey. Phone: 90 312 3051562. Fax: 90 312 3115250. E-mail: sarikan{at}metu.edu.tr.

Antimicrobial Agents and Chemotherapy, September 2002, p. 3084-3087, Vol. 46, No. 9
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.9.3084-3087.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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