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Antimicrobial Agents and Chemotherapy, January 2003, p. 181-187, Vol. 47, No. 1
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.1.181-187.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Proton Channel Is the Minimal Structure of ATP Synthase Necessary and Sufficient for Microcin H47 Antibiotic Action

Eliana Rodríguez and Magela Laviña*

Sección de Fisiología y Genética Bacterianas, Facultad de Ciencias, Montevideo, Uruguay

Received 14 April 2002/ Returned for modification 3 September 2002/ Accepted 8 October 2002

It had been previously determined that the presence of FoF1 ATP synthase was required for microcin H47 antibiotic action. In this work, microcin-resistant atp mutants were genetically analyzed. Their mutations, originated by Tn5 insertion, in all cases were found to affect determinants for the Fo portion of ATP synthase. To discern if microcin action required the presence of the entire complex or if the Fo proton channel would suffice, recombinant plasmids carrying different segments of the atp operon were constructed and introduced into an atp deletion strain. The phenotypic analysis of the strains thus obtained clearly indicated that the presence of the Fo proton channel was absolutely required for microcin H47 action, while the F1 catalytic portion was found to be dispensable. Furthermore, when any of the three components of the proton channel was missing, total resistance to the antibiotic ensued. Complementation analysis between atp::Tn5 chromosomal mutations and recombinant atp plasmid constructions further supported the idea that the proton channel would be the minimal structure of the ATP synthase complex needed for microcin H47 antibiotic action.

* Corresponding author. Mailing address: Sección de Fisiología y Genética Bacterianas, Facultad de Ciencias, Iguá 4225, Malvín Norte, Montevideo 11.400, Uruguay. Phone: (5982) 525 86 18, ext. 143. Fax: (5982) 525 86 29. E-mail: magela{at}fcien.edu.uy.

Antimicrobial Agents and Chemotherapy, January 2003, p. 181-187, Vol. 47, No. 1
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.1.181-187.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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