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Drug Targeting Mycobacterium tuberculosis Cell Wall Synthesis: Development of a Microtiter Plate-Based Screen for UDP-Galactopyranose Mutase and Identification of an Inhibitor from a Uridine-Based Library

Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523,¹ Departments of Chemistry and Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, California 94720²

Received 29 May 2002/ Returned for modification 19 August 2002/ Accepted 17 October 2002

A microtiter plate assay for UDP-galactopyranose mutase, an essential cell wall biosynthetic enzyme of Mycobacterium tuberculosis, was developed. The assay is based on the release of tritiated formaldehyde from UDP-galactofuranose but not UDP-galactopyranose by periodate and was used to identify a uridine-based enzyme inhibitor from a chemical library.

Present address: Department of Medicine, Division of Infectious Diseases, Stanford University School of Medicine, Stanford, CA 94305.

Present address: Heska Corporation, Fort Collins, CO 80525.

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