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Antimicrobial Agents and Chemotherapy, January 2003, p. 383-386, Vol. 47, No. 1
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.1.383-386.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

gyrA Mutations in Ciprofloxacin-Resistant Bartonella bacilliformis Strains Obtained In Vitro

Michael F. Minnick,* Zachary R. Wilson, Laura S. Smitherman, and D. Scott Samuels

Division of Biological Sciences, The University of Montana, Missoula, Montana 59812

Received 2 April 2002/ Returned for modification 5 August 2002/ Accepted 1 October 2002

We isolated and characterized mutants of Bartonella bacilliformis that are resistant to the fluoroquinolone antibiotic ciprofloxacin, which targets the A subunit of DNA gyrase. Mutants had single point mutations in the gyrA gene that changed either Asp-90 to Gly or Asp-95 to Asn and had 3- or 16-fold higher resistance, respectively, to ciprofloxacin than did wild-type B. bacilliformis. Asp-95 is homologous to Asp-87 of Escherichia coli GyrA and is a common residue mutated in fluoroquinolone-resistant strains of other bacteria. This is the first report of a mutation at an Asp-90 homologue, which corresponds to Asp-82 in E. coli GyrA.

* Corresponding author. Mailing address: Division of Biological Sciences, The University of Montana, Missoula, MT 59812-4824. Phone: (406) 243-5972. Fax: (406) 243-4184. E-mail: mike.minnick{at}mso.umt.edu.

Antimicrobial Agents and Chemotherapy, January 2003, p. 383-386, Vol. 47, No. 1
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.1.383-386.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Angelakis, E., Raoult, D., Rolain, J.-M. (2009). Molecular characterization of resistance to fluoroquinolones in Bartonella henselae and Bartonella quintana. J Antimicrob Chemother 63: 1288-1289 [Full Text]  
  • Angelakis, E., Biswas, S., Taylor, C., Raoult, D., Rolain, J.-M. (2008). Heterogeneity of susceptibility to fluoroquinolones in Bartonella isolates from Australia reveals a natural mutation in gyrA. J Antimicrob Chemother 61: 1252-1255 [Abstract] [Full Text]  
  • Biswas, S., Raoult, D., Rolain, J.-M. (2007). Molecular mechanisms of resistance to antibiotics in Bartonella bacilliformis. J Antimicrob Chemother 59: 1065-1070 [Abstract] [Full Text]  


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