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Antimicrobial Agents and Chemotherapy, January 2003, p. 390-394, Vol. 47, No. 1
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.1.390-394.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

In Vivo Selection of Campylobacter Isolates with High Levels of Fluoroquinolone Resistance Associated with gyrA Mutations and the Function of the CmeABC Efflux Pump

Naidan Luo, Orhan Sahin,1 Jun Lin, Linda O. Michel, and Qijing Zhang*

Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio 44691

Received 3 April 2002/ Returned for modification 20 May 2002/ Accepted 8 October 2002

Enrofloxacin treatment of chickens infected with fluoroquinolone(FQ)-sensitive Campylobacter promoted the emergence of FQ-resistant Campylobacter mutants which propagated in the intestinal tract and recolonized the chickens. The recovered isolates were highly resistant to quinolone antibiotics but remained susceptible to non-FQ antimicrobial agents. Specific single-point mutations in the gyrA gene and the function of the CmeABC efflux pump were linked to the acquired FQ resistance. These results reveal that Campylobacter is hypermutable in vivo under the selection pressure of FQ and highlight the need for the prudent use of FQ antibiotics.

* Corresponding author. Mailing address: Food Animal Health Research Program, Department of Veterinary Preventive Medicine, The Ohio State University, 1680 Madison Ave., Wooster, OH 44691. Phone: (330) 263-3747; Fax: (330) 263-3677. E-mail: zhang.234{at}osu.edu.

{dagger} Present address: Department of Microbiology, Veterinary Faculty, Mustafa Kemal University, Hatay, Turkey.

Antimicrobial Agents and Chemotherapy, January 2003, p. 390-394, Vol. 47, No. 1
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.1.390-394.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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