This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Braga, P. C.
Right arrow Articles by Dal Sasso, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Braga, P. C.
Right arrow Articles by Dal Sasso, M.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, January 2003, p. 408-412, Vol. 47, No. 1
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.1.408-412.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Flow Cytometric Assessment of Susceptibilities of Streptococcus pyogenes to Erythromycin and Rokitamycin

Pier Carlo Braga,* Cinzia Bovio, Maria Culici, and Monica Dal Sasso

Center of Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy

Received 1 March 2002/ Returned for modification 19 July 2002/ Accepted 9 September 2002

The effects of erythromycin (a 14-membered ring macrolide) and rokitamycin (a 16-membered ring macrolide) on the viability of the Streptococcus pyogenes M phenotype were studied by means of flow cytometry and fluorescence microscopy by using a combination of two fluorochromes (syto 9 and propidium iodide) that stains live bacteria green and dead bacteria red. In order to apply the flow cytometry, a bacterial sonication procedure was expressly set up to separate single cells from the long, intralaced S. pyogenes chains of up to 30 to 40 cells that have previously prevented the application of flow cytometry to this type of bacteria. The association of flow cytometry using an appropriate sonication procedure, together with a combination of fluorescent probes, offered the possibility of very quickly investigating the different microbiological effects of rokitamycin at 2 µg/ml, which was active on the S. pyogenes M phenotype, and of erythromycin at doses of up to 32 µg/ml, which was not.

* Corresponding author. Mailing address: Centro di Farmacologia Respiratoria, Via Vanvitelli 32, 20129 Milan, Italy. Phone: 39-02-5031 6990. Fax: 39-02-5031 6949. E-mail: bragapc{at}mailserver.unimi.it.

Antimicrobial Agents and Chemotherapy, January 2003, p. 408-412, Vol. 47, No. 1
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.1.408-412.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Assuncao, P., Antunes, N. T., Rosales, R. S., Poveda, C., Poveda, J. B., Davey, H. M. (2006). Flow Cytometric Determination of the Effects of Antibacterial Agents on Mycoplasma agalactiae, Mycoplasma putrefaciens, Mycoplasma capricolum subsp. capricolum, and Mycoplasma mycoides subsp. mycoides Large Colony Type.. Antimicrob. Agents Chemother. 50: 2845-2849 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»