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Antimicrobial Agents and Chemotherapy, January 2003, p. 432-435, Vol. 47, No. 1
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.1.432-435.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Lincomycin Resistance Mutations in Two Regions Immediately Downstream of the -10 Region of lmr Promoter Cause Overexpression of a Putative Multidrug Efflux Pump in Bacillus subtilis Mutants

Miyuki Kumano,1 Masaya Fujita,2 Kouji Nakamura,1 Makiko Murata,3 Reiko Ohki,3 and Kunio Yamane1*

Institute of Biological Sciences, University of Tsukuba, Tsukuba-shi, Ibaraki 305-8572,1 Department of Molecular Biology, School of Health Sciences, Kyorin University, Hachioji-shi, Tokyo 192-8508, Japan,3 Department of Molecular and Cellular Biology, Biological Laboratories, Harvard University, Cambridge, Massachusetts 023482

Received 16 May 2002/ Returned for modification 16 July 2002/ Accepted 3 October 2002

We isolated 19 lincomycin-resistant Bacillus subtilis mutants by expressing lmrB encoding a putative multidrug efflux protein. Eighteen of the mutants altered at two regions (-3 to -1 and +15) immediately downstream of the -10 region of the lmr promoter increased lmr transcription in vivo and in vitro.

* Corresponding author. Mailing address: Institute of Biological Sciences, University of Tsukuba, Tsukuba-shi, Ibaraki 305-8572, Japan. Phone: 81-298-53-6680. Fax: 81-298-53-6680. E-mail: kyamane{at}sakura.cc.tsukuba.ac.jp.

Antimicrobial Agents and Chemotherapy, January 2003, p. 432-435, Vol. 47, No. 1
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.1.432-435.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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