This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Collier, C. T.
Right arrow Articles by Gaskins, H. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Collier, C. T.
Right arrow Articles by Gaskins, H. R.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, October 2003, p. 3311-3317, Vol. 47, No. 10
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.10.3311-3317.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Effects of Tylosin on Bacterial Mucolysis, Clostridium perfringens Colonization, and Intestinal Barrier Function in a Chick Model of Necrotic Enteritis

C. T. Collier,1 J. D. van der Klis,2 B. Deplancke,3 D. B. Anderson,4 and H. R. Gaskins1,3,5*

Departments of Animal Sciences,1 Veterinary Pathobiology,5 Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois,3 Elanco Animal Health, Research and Development, Greenfield, Indiana,4 ID TNO Animal Nutrition, Lelystad, The Netherlands2

Received 3 July 2003/ Returned for modification 4 July 2003/ Accepted 18 July 2003

Necrotic enteritis (NE) is a worldwide poultry disease caused by the alpha toxin-producing bacterium Clostridium perfringens. Disease risk factors include concurrent coccidial infection and the dietary use of cereal grains high in nonstarch polysaccharides (NSP), such as wheat, barley, rye, and oats. Outbreaks of NE can be prevented or treated by the use of in-feed antibiotics. However, the current debate regarding the prophylactic use of antibiotics in animal diets necessitates a better understanding of factors that influence intestinal colonization by C. perfringens as well as the pathophysiological consequences of its growth. We report a study with a chick model of NE, which used molecular (16S rRNA gene [16S rDNA]) and culture-based microbiological techniques to investigate the impact of the macrolide antibiotic tylosin phosphate (100 ppm) and a dietary NSP (pectin) on the community structure of the small intestinal microbiota relative to colonization by C. perfringens. The effects of tylosin and pectin on mucolytic activity of the microbiota and C. perfringens colonization and their relationship to pathological indices of NE were of particular interest. The data demonstrate that tylosin reduced the percentage of mucolytic bacteria in general and the concentration of C. perfringens in particular, and these responses correlated in a temporal fashion with a reduction in the occurrence of NE lesions and an improvement in barrier function. The presence of pectin did not significantly affect the variables measured. Thus, it appears that tylosin can control NE through its modulation of C. perfringens colonization and the mucolytic activity of the intestinal microbiota.

* Corresponding author. Mailing address: University of Illinois at Urbana-Champaign, 1207 W. Gregory Dr., Urbana, IL 61801. Phone: (217) 244-3165. Fax: (217) 333-8804. E-mail: hgaskins{at}uiuc.edu.

Antimicrobial Agents and Chemotherapy, October 2003, p. 3311-3317, Vol. 47, No. 10
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.10.3311-3317.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhou, H., Gong, J., Brisbin, J., Yu, H., Sarson, A. J., Si, W., Sharif, S., Han, Y. (2009). Transcriptional profiling analysis of host response to Clostridium perfringens infection in broilers. Poult. Sci. 88: 1023-1032 [Abstract] [Full Text]  
  • Jia, W., Slominski, B. A., Bruce, H. L., Blank, G., Crow, G., Jones, O. (2009). Effects of diet type and enzyme addition on growth performance and gut health of broiler chickens during subclinical Clostridium perfringens challenge. Poult. Sci. 88: 132-140 [Abstract] [Full Text]  
  • Rehman, H., Ijaz, A., Specht, A., Dill, D., Hellweg, P., Manner, K., Zentek, J. (2009). In vitro effects of alpha toxin from Clostridium perfringens on the electrophysiological parameters of jejunal tissues from laying hens preincubated with inulin and N-acetyl-L-cysteine. Poult. Sci. 88: 199-204 [Abstract] [Full Text]  
  • Thompson, K., Burkholder, K., Patterson, J., Applegate, T. J. (2008). Microbial Ecology Shifts in the Ileum of Broilers During Feed Withdrawal and Dietary Manipulations. Poult. Sci. 87: 1624-1632 [Abstract] [Full Text]  
  • Santos, A. A. Jr., Ferket, P. R., Santos, F. B. O., Nakamura, N., Collier, C. (2008). Change in the Ileal Bacterial Population of Turkeys Fed Different Diets and After Infection with Salmonella as Determined with Denaturing Gradient Gel Electrophoresis of Amplified 16S Ribosomal DNA. Poult. Sci. 87: 1415-1427 [Abstract] [Full Text]  
  • Rehman, H., Hellweg, P., Taras, D., Zentek, J. (2008). Effects of Dietary Inulin on the Intestinal Short Chain Fatty Acids and Microbial Ecology in Broiler Chickens as Revealed by Denaturing Gradient Gel Electrophoresis. Poult. Sci. 87: 783-789 [Abstract] [Full Text]  
  • Zhou, H., Gong, J., Brisbin, J. T., Yu, H., Sanei, B., Sabour, P., Sharif, S. (2007). Appropriate Chicken Sample Size for Identifying the Composition of Broiler Intestinal Microbiota Affected by Dietary Antibiotics, Using the Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis Technique. Poult. Sci. 86: 2541-2549 [Abstract] [Full Text]  
  • Diarra, M. S., Silversides, F. G., Diarrassouba, F., Pritchard, J., Masson, L., Brousseau, R., Bonnet, C., Delaquis, P., Bach, S., Skura, B. J., Topp, E. (2007). Impact of Feed Supplementation with Antimicrobial Agents on Growth Performance of Broiler Chickens, Clostridium perfringens and Enterococcus Counts, and Antibiotic Resistance Phenotypes and Distribution of Antimicrobial Resistance Determinants in Escherichia coli Isolates. Appl. Environ. Microbiol. 73: 6566-6576 [Abstract] [Full Text]  
  • Wise, M. G., Siragusa, G. R. (2005). Quantitative Detection of Clostridium perfringens in the Broiler Fowl Gastrointestinal Tract by Real-Time PCR. Appl. Environ. Microbiol. 71: 3911-3916 [Abstract] [Full Text]  
  • Collier, C. T., Smiricky-Tjardes, M. R., Albin, D. M., Wubben, J. E., Gabert, V. M., Deplancke, B., Bane, D., Anderson, D. B., Gaskins, H. R. (2003). Molecular ecological analysis of porcine ileal microbiota responses to antimicrobial growth promoters. J ANIM SCI 81: 3035-3045 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»