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Antimicrobial Agents and Chemotherapy, October 2003, p. 3332-3335, Vol. 47, No. 10
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.10.3332-3335.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Role of Klebsiella pneumoniae OmpK35 Porin in Antimicrobial Resistance

Antonio Doménech-Sánchez,1,2 Luis Martínez-Martínez,3,4* Santiago Hernández-Allés,1,2 María del Carmen Conejo,3 Álvaro Pascual,3,4 Juan M. Tomás,5 Sebastián Albertí,1,2,6 and Vicente Javier Benedí1,2

Laboratory of Microbiology, Department of Biology, University of the Balearic Island,1 IMEDEA (CSIC-UIB),2 Research Unit, University Hospital Son Dureta, Palma de Mallorca,6 Department of Microbiology, School of Medicine, University of Seville,3 University Hospital V. Macarena, Seville,4 Department of Microbiology, University of Barcelona, Barcelona, Spain5

Received 25 February 2003/ Returned for modification 21 April 2003/ Accepted 12 July 2003

OmpK35 from Klebsiella pneumoniae is the homologue of Escherichia coli OmpF porin. Expression of OmpK35 in K. pneumoniae strain CSUB10R (lacking both OmpK35 and OmpK36) decreased the MICs of cephalosporins and meropenem >=128-fold and decreased the MICs of imipenem, ciprofloxacin, and chloramphenicol >=8-fold. MIC reductions by OmpK35 were 4 times (cefepime), 8 times (cefotetan, cefotaxime, and cefpirome), or 128 times (ceftazidime) higher than those caused by OmpK36, but the MICs were similar or 1 dilution lower for other evaluated agents.

* Corresponding author. Mailing address: Department of Microbiology, School of Medicine, University of Seville, Apdo. 914, 41080 Seville, Spain. Phone: 34-955008287. Fax: 34-954377413. E-mail: lmartin{at}us.es.

Antimicrobial Agents and Chemotherapy, October 2003, p. 3332-3335, Vol. 47, No. 10
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.10.3332-3335.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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