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Antimicrobial Agents and Chemotherapy, November 2003, p. 3415-3420, Vol. 47, No. 11
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.11.3415-3420.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Heterogeneity of Macrolide-Lincosamide-Streptogramin B Resistance Phenotypes in Enterococci

Yu-Hong Min,1 Jae-Hee Jeong,1 Yun-Jeong Choi,1 Hee-Jeong Yun,1 Kyungwon Lee,2 Mi-Ja Shim,3 Jin-Hwan Kwak,4 and Eung-Chil Choi1*

College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University,1 Department of Clinical Pathology and Research Institute of Bacterial Resistance, Yonsei University College of Medicine,2 Department of Life Science, The University of Seoul Seoul,3 School of Life & Food Sciences, Handong Global University, Pohang, Korea4

Received 15 January 2003/ Returned for modification 2 June 2003/ Accepted 31 July 2003

We determined the macrolide resistance phenotypes of 241 clinical isolates of erythromycin-resistant enterococci (MICs, >=1 µg/ml), including 147 Enterococcus faecalis strains and 94 Enterococcus faecium strains, collected from a hospital in Seoul, Korea, between 1999 and 2000. By the erythromycin (40 µg)-josamycin (100 µg) double-disk test, 93 strains were assigned to the constitutive macrolide, lincosamide, and streptogramin B (MLSB) resistance (cMLSB) phenotype, and the remaining 148 strains were assigned to the inducible MLSB resistance (iMLSB) phenotype. Of the strains with the iMLSB phenotype, 36 exhibited a reversibly inducible MLSB (riMLSB) phenotype, i.e., blunting of the erythromycin zone of inhibition, which indicates that the 16-membered-ring macrolide josamycin is a more effective inducer than the 14-membered-ring macrolide erythromycin. Sequence analysis of the regulatory regions of the erm(B) genes from all of the strains exhibiting the riMLSB phenotype revealed not only erm(Bv) [where v represents variant; previously erm(AMR)] (n = 13), as reported previously, but also three kinds of erm(B) variants, which were designated erm(Bv1) (n = 17), erm(Bv2) (n = 3), and erm(Bv3) (n = 3), respectively. In lacZ reporter gene assays of these variants, the 16-membered-ring macrolide tylosin had stronger inducibility than erythromycin at >=0.1 µg/ml. These findings highlight the versatility of erm(B) in induction specificity.

* Corresponding author. Mailing address: College of Pharmacy, Seoul National University, San 56-1, Shillim-Dong, Kwanak-Gu, Seoul 151-742, Korea. Phone: 82-2-880-7874. Fax: 82-2-886-5802. E-mail: ecchoi{at}snu.ac.kr.

Antimicrobial Agents and Chemotherapy, November 2003, p. 3415-3420, Vol. 47, No. 11
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.11.3415-3420.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Min, Y.-H., Kwon, A.-R., Yoon, E.-J., Shim, M.-J., Choi, E.-C. (2008). Translational Attenuation and mRNA Stabilization as Mechanisms of erm(B) Induction by Erythromycin. Antimicrob. Agents Chemother. 52: 1782-1789 [Abstract] [Full Text]  
  • Bailey, M., Chettiath, T., Mankin, A. S. (2008). Induction of erm(C) Expression by Noninducing Antibiotics. Antimicrob. Agents Chemother. 52: 866-874 [Abstract] [Full Text]  
  • Yoon, E.-J., Kwon, A.-R., Min, Y.-H., Choi, E.-C. (2008). Foggy D-shaped zone of inhibition in Staphylococcus aureus owing to a dual character of both inducible and constitutive resistance to macrolide-lincosamide-streptogramin B. J Antimicrob Chemother 61: 533-540 [Abstract] [Full Text]  
  • Madsen, C. T., Jakobsen, L., Douthwaite, S. (2005). Mycobacterium smegmatis Erm(38) Is a Reluctant Dimethyltransferase. Antimicrob. Agents Chemother. 49: 3803-3809 [Abstract] [Full Text]  


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