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Antimicrobial Agents and Chemotherapy, November 2003, p. 3653-3656, Vol. 47, No. 11
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.11.3653-3656.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

G484S Amino Acid Substitution in Lanosterol 14-{alpha} Demethylase (ERG11) Is Related to Fluconazole Resistance in a Recurrent Cryptococcus neoformans Clinical Isolate

Laura Rodero,1* Emilia Mellado,2 A. Carolina Rodriguez,1 Angela Salve,1 Liliana Guelfand,3 Pedro Cahn,3 Manuel Cuenca-Estrella,2 Graciela Davel,1 and Juan L. Rodriguez-Tudela2

Departamento Micología, Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán,",1 Hospital J. A. Fernandez, Buenos Aires, Argentina,3 Unidad de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Spain2

Received 2 May 2003/ Returned for modification 10 June 2003/ Accepted 11 August 2003

Five sequential Cryptococcus neoformans isolates recovered from an AIDS patient with recurrent meningitis were analyzed. Four isolates were fluconazole susceptible, while the fifth isolate developed fluconazole resistance. Analysis of the 14-{alpha} lanosterol demethylase gene (ERG11) showed a point mutation in the resistant strain responsible for the amino acid substitution G484S.

* Corresponding author. Mailing address: Departamento Micología, Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán," Av. Velez Sarsfield 563, (1281) Buenos Aires, Argentina. Phone and fax: 54-11-4302-5066. E-mail: lrodero{at}anlis.gov.ar.

Antimicrobial Agents and Chemotherapy, November 2003, p. 3653-3656, Vol. 47, No. 11
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.11.3653-3656.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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