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Antimicrobial Agents and Chemotherapy, December 2003, p. 3719-3723, Vol. 47, No. 12
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.12.3719-3723.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Epidemiology of Nalidixic Acid Resistance and TEM-1- and TEM-52-Mediated Ampicillin Resistance of Shigella sonnei Isolates Obtained in Korea between 1980 and 2000

Young Sook Jeong,1 Je Chul Lee,2 Hee Young Kang,1 Hak Sun Yu,1 Eun Young Lee,1 Chul Hee Choi,1 Seong Ho Tae,1 Yoo Chul Lee,1 Dong Taek Cho,1 and Sung Yong Seol1*

Department of Microbiology, Kyungpook National University School of Medicine, Taegu 700-422,1 Department of Microbiology, Eulji University School of Medicine, Taejeon 301-832,Korea2

Received 8 May 2003/ Returned for modification 3 July 2003/ Accepted 19 August 2003

The resistance to ampicillin and nalidixic acid in Shigella sonnei isolates obtained in Korea during the period 1998 to 2000 was characterized. Recently (J. Y. Oh, H. S. Yu, S. K. Kim, S. Y. Seol, D. T. Cho, and J. C. Lee, J. Clin. Microbiol. 41:421-423, 2003) ampicillin and nalidixic acid resistance was found in 49 and 70%, respectively, of the 67 S. sonnei isolates obtained during this period. We analyzed 138 S. sonnei isolates collected during the same period. Ampicillin and nalidixic acid resistance was found in 30 and 86% of the isolates, respectively. The ampicillin resistance was mediated by a TEM-1 ß-lactamase, and TEM-52 extended-spectrum ß-lactamase was identified in one sporadic S. sonnei isolate from 1999. blaTEM-1 and blaTEM-52 were located in conjugative R-plasmids. Tn3 was detected in 41% of the ampicillin-resistant isolates. The R-plasmids from the transconjugants that transferred resistance to ampicillin exhibited different restriction fragment length polymorphism patterns, and a blaTEM-1 probe was hybridized with the different fragments. The nalidixic acid resistance was exclusively associated with an amino acid substitution, Ser83->Leu (TCG->TTG), in gyrA. These findings indicate that the genetically related S. sonnei strains readily acquire resistance to ampicillin, streptomycin, trimethoprim, and sulfamethoxazole but not nalidixic acid through conjugative R-plasmids from difference sources when confronted by antibiotic selective pressures.

* Corresponding author. Mailing address: Department of Microbiology, Kyungpook National University School of Medicine, 101 Dongin-dong, Chung-gu, Taegu 700-422, Korea. Phone: 82-53-420-6952. Fax: 82-53-427-5664. E-mail: syseol{at}knu.ac.kr.

Antimicrobial Agents and Chemotherapy, December 2003, p. 3719-3723, Vol. 47, No. 12
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.12.3719-3723.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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