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Antimicrobial Agents and Chemotherapy, December 2003, p. 3724-3732, Vol. 47, No. 12
0066-4804/03/$08.00+0 DOI: 10.1128/AAC.47.12.3724-3732.2003
Copyright © 2003, American
Society for
Microbiology. All Rights Reserved.
Prevalence and Molecular Epidemiology of CTX-M Extended-Spectrum ß-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Russian Hospitals
M. Edelstein,* M. Pimkin, I. Palagin, I. Edelstein, and L. Stratchounski
Institute
of Antimicrobial Chemotherapy, Smolensk State Medical
Academy, Smolensk 214019, Russia
Received 31 March 2003/
Returned for modification 7 July 2003/
Accepted 8 September 2003
A
total of 904 consecutive nosocomial isolates of Escherichia
coli and Klebsiella pneumoniae collected from 28 Russian
hospitals were screened for production of extended-spectrum
ß-lactamases (ESBLs). The ESBL phenotype was detected in 78
(15.8%) E. coli and 248 (60.8%) K.
pneumoniae isolates. One hundred fifteen isolates carried the
genes for CTX-M-type ß-lactamases, which, as shown by
PCR-restriction fragment length polymorphism analysis, were distributed
into the two genetic groups of CTX-M-1 (93%)- and CTX-M-2
(7%)-related enzymes. Isolates producing the enzymes of the
first group were found in 20 hospitals from geographically distant
regions of the country and were characterized by considerable diversity
of genetic types, as was demonstrated by enterobacterial repetitive
consensus PCR typing. Within this group the CTX-M-3 and the CTX-M-15
ß-lactamases were identified. In contrast, the enzymes of the
CTX-M-2 group (namely, CTX-M-5) were detected only in eight clonally
related E. coli isolates from a single hospital. Notably, the
levels of resistance to ceftazidime were remarkably variable among the
CTX-M producers. This study provides further evidence of the global
dissemination of CTX-M type ESBLs and emphasizes the need for their
epidemiological
monitoring.
* Corresponding
author. Mailing address: Institute of Antimicrobial Chemotherapy,
Smolensk State Medical Academy, P.O. Box 5, 28, Krupskaya St., Smolensk
214019, Russia. Phone: 7-0812-611301. Fax: 7-0812-611294. E-mail:
me{at}antibiotic.ru.
Antimicrobial Agents and Chemotherapy, December 2003, p. 3724-3732, Vol. 47, No. 12
0066-4804/03/$08.00+0 DOI: 10.1128/AAC.47.12.3724-3732.2003
Copyright © 2003, American
Society for
Microbiology. All Rights Reserved.
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