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Antimicrobial Agents and Chemotherapy, December 2003, p. 3764-3767, Vol. 47, No. 12
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.12.3764-3767.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Increased Killing of Staphylococci and Streptococci by Daptomycin Compared with Cefazolin and Vancomycin in an In Vitro Peritoneal Dialysate Model

Elizabeth D. Hermsen,1 Laurie B. Hovde,1 John R. Hotchkiss,2 and John C. Rotschafer1*

Department of Experimental and Clinical Pharmacology, The University of Minnesota College of Pharmacy, Minneapolis,1 Section of Nephrology, Regions Hospital, St. Paul, Minnesota2

Received 16 July 2003/ Returned for modification 16 August 2003/ Accepted 31 August 2003

Peritoneal dialysate fluid (PDF) is a bacteriostatic medium that compromises the antibacterial activity of cell wall-active agents. By use of an in vitro static model, methicillin-resistant Staphylococcus aureus (MRSA), methicillin-susceptible S. aureus (MSSA), methicillin-susceptible Staphylococcus epidermidis (MSSE), and Streptococcus sanguis were exposed to daptomycin at concentrations of 10, 30, and 100 mg/liter, cefazolin at 125 mg/liter, and vancomycin at 25 mg/liter in cation-adjusted Mueller-Hinton Broth or Todd Hewitt Broth (for S. sanguis) and PDF at pHs of 5.5 and 7.4. The pH had no effect on antibacterial activity. Neither cefazolin nor vancomycin produced a bactericidal or a bacteriostatic effect versus MRSA, MSSA, MSSE, or S. sanguis in PDF, while all concentrations of daptomycin were bactericidal against all organisms in PDF. Daptomycin did not exhibit concentration-dependent activity in PDF. Daptomycin appears to be a promising agent for use in peritoneal dialysis-associated peritonitis, producing bacterial kill to a greater extent and at a higher rate than cefazolin or vancomycin in PDF.

* Corresponding author. Mailing address: University of Minnesota College of Pharmacy, Department of Experimental and Clinical Pharmacology, 7-170 Weaver-Densford Hall, 308 Harvard St. SE, Minneapolis, MN 55455. Phone: (612) 624-2183. Fax: (612) 625-1140. E-mail: rotsc001{at}umn.edu.

Antimicrobial Agents and Chemotherapy, December 2003, p. 3764-3767, Vol. 47, No. 12
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.12.3764-3767.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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