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Antimicrobial Agents and Chemotherapy, December 2003, p. 3950-3953, Vol. 47, No. 12
0066-4804/03/$08.00+0 DOI: 10.1128/AAC.47.12.3950-3953.2003
Copyright © 2003, American
Society for
Microbiology. All Rights Reserved.
First Department of Propaedeutic Medicine,1 First Department of Pediatrics, Athens University, Athens,2 Department of Clinical Microbiology, Metaxa Hospital, Piraeus,Greece,5 Department of Medicine, William Beaumont Hospital, Royal Oak,3 Division of Infectious Diseases, Wayne State University School of Medicine,Detroit, Michigan4
Received 17 April 2003/ Returned for modification 28 July 2003/ Accepted 4 September 2003
The elements conferring high-level gentamicin resistance in 64 clinical isolates of Enterococcus faecalis were characterized by PCR and by restriction enzyme hybridization analysis of genomic and plasmid DNA. There was a strong association between gentamicin resistance and the aac(6')-aph(2") gene carried on IS256-based elements with different structures, locations, and transfer characteristics.
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