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Antimicrobial Agents and Chemotherapy, February 2003, p. 755-758, Vol. 47, No. 2
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.2.755-758.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Emergence in Klebsiella pneumoniae of a Chromosome-Encoded SHV ß-Lactamase That Compromises the Efficacy of Imipenem

Laurent Poirel,1 Claire Héritier,1 Isabelle Podglajen,2 Wladimir Sougakoff,2 Laurent Gutmann,2 and Patrice Nordmann1*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Le Kremlin-Bicêtre,1 Laboratoire de Recherche Moléculaire sur les Antibiotiques, INSERM, Université Paris VI, Paris, France2

Received 19 June 2002/ Returned for modification 8 September 2002/ Accepted 10 November 2002

A Klebsiella pneumoniae isolate was identified that had reduced susceptibility to several expanded-spectrum cephalosporins and imipenem. That isolate produced a chromosome-encoded SHV-type ß-lactamase, SHV-38, that had an alanine to valine substitution in position Ambler 146 compared to ß-lactamase SHV-1. The kinetic parameters for purified ß-lactamases SHV-38 and SHV-1 showed that the hydrolytic spectrum of SHV-38 included only ceftazidime and imipenem. This report is the first example of an SHV-type ß-lactamase capable of hydrolyzing imipenem.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78, Rue du Général Leclerc, 94275 Le Kremlin-Bicêtre, France. Phone: 33 1 45 21 36 32. Fax: 33 1 45 21 63 40. E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, February 2003, p. 755-758, Vol. 47, No. 2
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.2.755-758.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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