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Antimicrobial Agents and Chemotherapy, March 2003, p. 1062-1067, Vol. 47, No. 3
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.3.1062-1067.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Amino Acid Sequence Requirements at Residues 69 and 238 for the SME-1 ß-Lactamase To Confer Resistance to ß-Lactam Antibiotics

Verna and Marrs McLean Department of Biochemistry and Molecular Biology,¹ Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030²

Received 4 September 2002/ Returned for modification 24 October 2002/ Accepted 6 December 2002

Carbapenem antibiotics have been used to counteract resistant strains of bacteria harboring ß-lactamases and extended-spectrum ß-lactamases. Four enzymes from the class A group of ß-lactamases, NMC-A, IMI-1, SME-1, and KPC-1, efficiently hydrolyze carbapenem antibiotics. Sequence comparisons and structural information indicate that cysteines at amino acid residues 69 and 238, which are conserved in all four of these enzymes, form a disulfide bond that is unique to these ß-lactamases. To test whether this disulfide bond is required for catalytic activity, the codons for residues Cys69 and Cys238 were randomized individually and simultaneously by PCR-based mutagenesis to create random replacement libraries for these positions. Mutants that were able to confer resistance to ampicillin, imipenem, or cefotaxime were selected from these libraries. The results indicate that positions Cys69 and Cys238 are critical for hydrolysis of all of the antibiotics tested, suggesting that the disulfide bond is generally required for this enzyme to catalyze the hydrolysis of ß-lactam antibiotics.

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