This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vacher, S.
Right arrow Articles by Mégraud, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vacher, S.
Right arrow Articles by Mégraud, F.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, March 2003, p. 1125-1128, Vol. 47, No. 3
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.3.1125-1128.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

PCR-Restriction Fragment Length Polymorphism Analysis for Detection of Point Mutations Associated with Macrolide Resistance in Campylobacter spp.

Sylvie Vacher, Armelle Ménard,* Elisabeth Bernard, and Francis Mégraud

Centre National de Référence des Helicobacters et Campylobacters, Laboratoire de Bactériologie, Hopital Pellegrin, and Laboratoire de Bactériologie, Université Victor Segalen Bordeaux 2, Bordeaux, France

Received 13 September 2002/ Returned for modification 25 November 2002/ Accepted 20 December 2002

A 23S rRNA gene fragment in domain V was sequenced from 30 clinical isolates of Campylobacter spp., including 22 resistant to macrolides. Two point mutations associated with erythromycin resistance were identified at positions 2074 and 2075 on the 23S rRNA gene (homologous to A2142C and A2143G mutations in Helicobacter pylori) in which an adenine residue is also replaced with a cytosine and a guanine residue, respectively. A combined PCR-restriction fragment length polymorphism technique was developed to detect these mutations by using the BsaI and BceAI enzymes.

* Corresponding author. Mailing address: Laboratoire de Bactériologie, Zone Nord, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France. Phone: 33 5-57-57-12-88. Fax: 33 5-56-79-60-18. E-mail: armelle.menard{at}labhel.u-bordeaux2.fr.

Antimicrobial Agents and Chemotherapy, March 2003, p. 1125-1128, Vol. 47, No. 3
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.3.1125-1128.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Korczak, B. M., Zurfluh, M., Emler, S., Kuhn-Oertli, J., Kuhnert, P. (2009). Multiplex Strategy for Multilocus Sequence Typing, fla Typing, and Genetic Determination of Antimicrobial Resistance of Campylobacter jejuni and Campylobacter coli Isolates Collected in Switzerland. J. Clin. Microbiol. 47: 1996-2007 [Abstract] [Full Text]  
  • Kim, J.-S., Kim, J.-W., Kathariou, S. (2008). Differential Effects of Temperature on Natural Transformation to Erythromycin and Nalidixic Acid Resistance in Campylobacter coli. Appl. Environ. Microbiol. 74: 6121-6125 [Abstract] [Full Text]  
  • Belanger, A. E., Shryock, T. R. (2007). Macrolide-resistant Campylobacter: the meat of the matter. J Antimicrob Chemother 60: 715-723 [Abstract] [Full Text]  
  • Gibreel, A., Wetsch, N. M., Taylor, D. E. (2007). Contribution of the CmeABC Efflux Pump to Macrolide and Tetracycline Resistance in Campylobacter jejuni. Antimicrob. Agents Chemother. 51: 3212-3216 [Abstract] [Full Text]  
  • Gibreel, A., Taylor, D. E. (2006). Macrolide resistance in Campylobacter jejuni and Campylobacter coli. J Antimicrob Chemother 58: 243-255 [Abstract] [Full Text]  
  • Kim, J.-S., Carver, D. K., Kathariou, S. (2006). Natural Transformation-Mediated Transfer of Erythromycin Resistance in Campylobacter coli Strains from Turkeys and Swine. Appl. Environ. Microbiol. 72: 1316-1321 [Abstract] [Full Text]  
  • Mamelli, L., Prouzet-Mauleon, V., Pages, J.-M., Megraud, F., Bolla, J.-M. (2005). Molecular basis of macrolide resistance in Campylobacter: role of efflux pumps and target mutations. J Antimicrob Chemother 56: 491-497 [Abstract] [Full Text]  
  • Gibreel, A., Kos, V. N., Keelan, M., Trieber, C. A., Levesque, S., Michaud, S., Taylor, D. E. (2005). Macrolide Resistance in Campylobacter jejuni and Campylobacter coli: Molecular Mechanism and Stability of the Resistance Phenotype. Antimicrob. Agents Chemother. 49: 2753-2759 [Abstract] [Full Text]  
  • Haanpera, M., Huovinen, P., Jalava, J. (2005). Detection and Quantification of Macrolide Resistance Mutations at Positions 2058 and 2059 of the 23S rRNA Gene by Pyrosequencing. Antimicrob. Agents Chemother. 49: 457-460 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»