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Antimicrobial Agents and Chemotherapy, April 2003, p. 1285-1290, Vol. 47, No. 4
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.4.1285-1290.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Integron-Containing IncU R Plasmids pRAS1 and pAr-32 from the Fish Pathogen Aeromonas salmonicida

Henning Sørum,* Trine M. L'Abée-Lund, Asta Solberg, and Anette Wold

Department of Pharmacology, Microbiology, and Food Hygiene, Norwegian School of Veterinary Science, Oslo, Norway

Received 13 March 2002/ Returned for modification 5 June 2002/ Accepted 3 January 2003

A 45-kb R plasmid, pRAS1, that confers resistance to tetracyclines, trimethoprim, and sulfonamides was isolated in 1989 from an atypical strain of the fish pathogen Aeromonas salmonicida. This plasmid could be transferred by conjugation to Escherichia coli with a high degree of efficiency (frequency, 0.48). The following year pRAS1 was isolated from A. salmonicida subsp. salmonicida in the same area. Incompatibility group U plasmid pRAS1 contained a drug resistance-determining region of 12 kb consisting of a class 1 integron similar to In4 of Tn1696 but with a dfrA16 gene cassette inserted. Close to IS6100 at the right end of Tn4 was a truncated Tn1721. Restriction enzyme analysis showed that R plasmid pAr-32, isolated from A. salmonicida in Japan in 1970, had the same backbone structure as pRAS1, while the drug resistance-determining region contained a complex class 1 integron with an aadA2 cassette; the chloramphenicol resistance gene catA2, as in In6 of pSa; and a duplicate of the 3' conserved segment of the integron.

* Corresponding author. Mailing address: Department of Pharmacology, Microbiology, and Food Hygiene, Norwegian School of Veterinary Science, Post Box 8146 Dep., 0033 Oslo, Norway. Phone: 4722964770. Fax: 4722964818. E-mail: Henning.Sorum{at}veths.no.

Antimicrobial Agents and Chemotherapy, April 2003, p. 1285-1290, Vol. 47, No. 4
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.4.1285-1290.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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