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Antimicrobial Agents and Chemotherapy, May 2003, p. 1647-1651, Vol. 47, No. 5
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.5.1647-1651.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Correlation between E-Test, Disk Diffusion, and Microdilution Methods for Antifungal Susceptibility Testing of Fluconazole and Voriconazole

Madonna J. Matar,* Luis Ostrosky-Zeichner, Victor L. Paetznick, Jose R. Rodriguez, Enuo Chen, and John H. Rex

Laboratory of Mycology Research, Division of Infectious Diseases, University of Texas—Houston Medical School, Houston, Texas 77030

Received 29 October 2002/ Returned for modification 13 November 2002/ Accepted 31 January 2003

The activities of fluconazole and voriconazole against isolates of Candida spp. (n = 400) were tested by the E-test, disk diffusion, and the National Committee for Clinical Laboratory Standards (NCCLS) M27-A2 broth microdilution-based reference methods. More than 96% of isolates found to be susceptible to fluconazole by the reference method were identified as susceptible by the agar-based methods. Lesser degrees of correlation with the reference method were seen for isolates identified as resistant by the agar-based methods. Interpretive categories are not available for voriconazole, but results qualitatively similar to those for fluconazole were seen. The agar-based E-test and disk diffusion methods are reliable alternatives to the NCCLS M27-A2 reference microdilution method for isolates that test susceptible to fluconazole.

* Corresponding author. Present address: Faculty of Medical Sciences, Lebanese University, P.O. Box 55564 Sin-El-Fil, Beirut, Lebanon. E-mail: madonne{at}inco.com.lb.

Antimicrobial Agents and Chemotherapy, May 2003, p. 1647-1651, Vol. 47, No. 5
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.5.1647-1651.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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