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Antimicrobial Agents and Chemotherapy, June 2003, p. 2002-2005, Vol. 47, No. 6
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.6.2002-2005.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evolution of Antibiotic Resistance in Salmonella enterica Serovar Typhimurium Strains Isolated in the Czech Republic between 1984 and 2002

Veterinary Research Institute, 621 32 Brno,¹ National Institute of Public Health, Center for Food Chain Hygiene,² University of Veterinary and Pharmaceutical Sciences, 612 42 Brno, Czech Republic³

Received 9 December 2002/ Returned for modification 30 January 2003/ Accepted 26 February 2003

In a collection of 66 Salmonella enterica serovar Typhimurium strains isolated between 1984 and 2002 in the Czech Republic, genes coding for antibiotic resistance were determined by using specific PCRs. We found that the pentadrug-resistant ACSSuT clone first appeared in the Czech Republic in 1990. A new variant of the aadA gene designated aadA21 is described, the 5' end of which was identical to aadA2 and the 3' end of which was identical to aadA1.

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