Previous Article | Next Article 
Antimicrobial Agents and Chemotherapy, July 2003, p. 2093-2099, Vol. 47, No. 7
0066-4804/03/$08.00+0 DOI: 10.1128/AAC.47.7.2093-2099.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
Correlation between Resistance of Pseudomonas aeruginosa to Quaternary Ammonium Compounds and Expression of Outer Membrane Protein OprR
Atsushi Tabata,1 Hideaki Nagamune,1 Takuya Maeda,1 Keiji Murakami,2 Yoichiro Miyake,2 and Hiroki Kourai1*Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, Tokushima 770-8506,1 Department of Oral Microbiology, School of Dentistry, The University of Tokushima, Tokushima 770-8504, Japan2
Received 24 October 2002/ Returned for modification 16 January 2003/ Accepted 7 April 2003
The adaptation mechanism of Pseudomonas aeruginosa ATCC 10145 to quaternary ammonium compounds (QACs) was investigated. A P. aeruginosa strain with adapted resistance to QACs was developed by a standard broth dilution method. It was revealed that P. aeruginosa exhibited remarkable resistance to N-dodecylpyridinium iodide (P-12), whose structure is similar to that of a common disinfectant, cetylpyridinium chloride. Adapted resistance to benzalkonium chloride (BAC), which is commonly used as a disinfectant, was also observed in P. aeruginosa. Moreover, the P-12-resistant strain exhibited cross-resistance to BAC. Analysis of the outer membrane protein of the P-12-resistant strain by two-dimensional polyacrylamide gel electrophoresis showed a significant increase in the level of expression of a protein (named OprR) whose molecular mass was approximately 26 kDa. The actual function of OprR is not yet clear; however, OprR was expected to be an outer membrane-associated protein with homology to lipoproteins of other bacterial species, according to a search of the National Center for Biotechnology Information website with the BLAST program by use of the N-terminal sequence of OprR. A correlation between the level of expression of OprR and the level of resistance of P. aeruginosa to QACs was observed by using a PA2800 gene knockout mutant derived from the P-12-resistant strain. The knockout mutant recovered susceptibility not only to P-12 but also to BAC. These results suggested that OprR significantly participated in the adaptation of P. aeruginosa to QACs, such as P-12 and BAC.
* Corresponding author. Mailing address: Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, No. 1, 2-chome, Minamijosanjima-cho, Tokushima 770-8506, Japan. Phone: 81-88-656-7408. Fax: 81-88-656-9148. E-mail: kourai{at}bio.tokushima-u.ac.jp.
Antimicrobial Agents and Chemotherapy, July 2003, p. 2093-2099, Vol. 47, No. 7
0066-4804/03/$08.00+0 DOI: 10.1128/AAC.47.7.2093-2099.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Maseda, H., Hashida, Y., Konaka, R., Shirai, A., Kourai, H.
(2009). Mutational Upregulation of a Resistance-Nodulation-Cell Division-Type Multidrug Efflux Pump, SdeAB, upon Exposure to a Biocide, Cetylpyridinium Chloride, and Antibiotic Resistance in Serratia marcescens. Antimicrob. Agents Chemother.
53: 5230-5235
[Abstract] [Full Text] -
Mangalappalli-Illathu, A. K., Korber, D. R.
(2006). Adaptive Resistance and Differential Protein Expression of Salmonella enterica Serovar Enteritidis Biofilms Exposed to Benzalkonium Chloride. Antimicrob. Agents Chemother.
50: 3588-3596
[Abstract] [Full Text] -
McBain, A. J., Ledder, R. G., Moore, L. E., Catrenich, C. E., Gilbert, P.
(2004). Effects of Quaternary-Ammonium-Based Formulations on Bacterial Community Dynamics and Antimicrobial Susceptibility. Appl. Environ. Microbiol.
70: 3449-3456
[Abstract] [Full Text]
Copyright © 2009 by the American Society for Microbiology. For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»