This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Givens, M. D. Articles by Boykin, D. W. Search for Related Content PubMed PubMed Citation Articles by Givens, M. D. Articles by Boykin, D. W.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, July 2003, p. 2223-2230, Vol. 47, No. 7
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.7.2223-2230.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of Inhibition of Bovine Viral Diarrhea Virus by Aromatic Cationic Molecules

Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama 36849 ,¹ Department of Chemistry, Georgia State University, Atlanta, Georgia 30303²

Received 8 October 2002/ Returned for modification 12 December 2002/ Accepted 24 March 2003

Bovine viral diarrhea virus (BVDV) is an economically significant pathogen of cattle and a problematic contaminant in the laboratory. BVDV is often used as an in vitro model for hepatitis C virus during drug discovery efforts. Aromatic dicationic molecules have exhibited inhibitory activity against several RNA viruses. Thus, the purpose of this research was to develop and apply a method for screening the aromatic cationic compounds for in vitro cytotoxicity and activity against a noncytopathic strain of BVDV. The screening method evaluated the concentration of BVDV in medium and cell lysates after 72 h of cell culture in the presence of either a 25 or 5 µM concentration of the test compound. Five of 93 screened compounds were selected for further determination of inhibitory (90 and 50%) and cytotoxic (50 and 10%) concentration endpoints. The screening method identified compounds that exhibited inhibition of BVDV at nanomolar concentrations while exhibiting no cytotoxicity at 25 µM concentrations. The leading compounds require further investigation to determine their mechanism of action, in vivo activity, and specific activity against hepatitis C virus.

This article has been cited by other articles:

• Paeshuyse, J., Leyssen, P., Mabery, E., Boddeker, N., Vrancken, R., Froeyen, M., Ansari, I. H., Dutartre, H., Rozenski, J., Gil, L. H. V. G., Letellier, C., Lanford, R., Canard, B., Koenen, F., Kerkhofs, P., Donis, R. O., Herdewijn, P., Watson, J., De Clercq, E., Puerstinger, G., Neyts, J. (2006). A Novel, Highly Selective Inhibitor of Pestivirus Replication That Targets the Viral RNA-Dependent RNA Polymerase. J. Virol. 80: 149-160 [Abstract] [Full Text]