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Antimicrobial Agents and Chemotherapy, July 2003, p. 2316-2318, Vol. 47, No. 7
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.7.2316-2318.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of Nucleotide Variability in rpoB in both Rifampin-Sensitive and Rifampin-Resistant Strains of Chlamydia trachomatis

Ute Dreses-Werringloer,1 Ingrid Padubrin,2 Lars Köhler,2 and Alan P. Hudson1*

Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan,1 Department of Internal Medicine, Division of Rheumatology, Medical School Hannover, Hannover, Germany2

Received 30 September 2002/ Returned for modification 17 January 2003/ Accepted 5 April 2003

A 656-bp PCR fragment from rpoB was sequenced from five rifampin-resistant Chlamydia trachomatis variants selected in vitro from a wild-type parent with a surprising level of genetic variability in this region. Three variants (MIC, 4 µg/ml) showed Ala522->Val in cluster I (codons 507 to 533), which harbors mutations in most rifampin-resistant bacteria. Two high-level resistance variants (MICs, 64 and 256 µg/ml) showed His526->Tyr in cluster I with additional genetic variation, some of which resulted in amino acid substitutions. None of the latter was situated in clusters related to rifampin resistance in other bacteria.

* Corresponding author. Mailing address: Department of Immunology and Microbiology, Wayne State University School of Medicine, Gordon H. Scott Hall, 540 E. Canfield Ave., Detroit, MI 48201. Phone: (313) 993-6641. Fax: (313) 577-1155. E-mail: ahudson{at}med.wayne.edu.

Antimicrobial Agents and Chemotherapy, July 2003, p. 2316-2318, Vol. 47, No. 7
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.7.2316-2318.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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