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Antimicrobial Agents and Chemotherapy, August 2003, p. 2579-2583, Vol. 47, No. 8
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.8.2579-2583.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Transferable vanB2 Tn5382-Containing Elements in Fecal Streptococcal Strains from Veal Calves

Kristin H. Dahl1* and Arnfinn Sundsfjord1,2*

Department of Microbiology and Virology, University of Tromsø,1 University Hospital of North-Norway, Tromsø, Norway2

Received 18 March 2003/ Returned for modification 18 April 2003/ Accepted 22 May 2003

Three vancomycin-resistant veal calf fecal streptococci, identified as Streptococcus gallolyticus (n = 2) and Streptococcus lutetiensis, were shown to harbor vanB2 Tn5382-like elements earlier described in enterococci. One S. gallolyticus strain had a 1,495-bp IS256-related element inserted in vanSB. The vanB2 Tn5382 element present in the plasmid-free S. lutetiensis strain was transferable to Enterococcus faecium BM4105-RF, Enterococcus faecalis JH2-2, and its recombination-deficient derivative, UV202. The transfer frequencies were comparable between recipient strains (from 1 x 10-7 to 7 x 10-6). All transconjugants acquired a vanB-containing chromosomal insert of approximately 100 kb, apparently by site-specific integration. Secondary transconjugants were not observed in intraspecies retransfer experiments. These observations are consistent with a conjugative, selftransmissible, integrative element that might be involved in the interspecies spread of vanB2 resistance determinants. Two JH2-2-derived transconjugants had also gained additional copies of large vanB-containing chromosomal fragments, a process that involves unexplained mechanisms that seems to require functional host cell-dependent recombination mechanisms.

* Corresponding author. Mailing address: Department of Microbiology and Virology, Institute for Medical Biology, University of Tromsø, N-9037 Tromsø, Norway. Phone for Kristin Dahl: 47 77 64 57 52. Phone for Arnfinn Sundsfjord: 47 77 64 62 02. Fax: 47 77 64 53 50. E-mail for Kristin Dahl: kristind{at}fagmed.uit.no. E-mail for Arnfinn Sundsfjord: arnfinns{at}fagmed.uit.no.

Antimicrobial Agents and Chemotherapy, August 2003, p. 2579-2583, Vol. 47, No. 8
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.8.2579-2583.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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