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Antimicrobial Agents and Chemotherapy, August 2003, p. 2669-2673, Vol. 47, No. 8
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.8.2669-2673.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular and Biochemical Characterization of a Novel Class A ß-Lactamase (HER-1) from Escherichia hermannii

Anne Beauchef-Havard,1 Guillaume Arlet,2,3* Valerie Gautier,3 Roger Labia,4 Patrick Grimont,5 and Alain Philippon1

Service de Bactériologie, Hôpital Cochin, AP-HP,1 Service de Bactériologie, Hôpital Tenon, AP-HP,2 EA 2392, UFR Saint-Antoine,3 Unité des Entérobactéries, INSERM U389, Institut Pasteur, Paris,5 CNRS-UBO-MHN, Unité FRE 2125, Quimper, France4

Received 10 March 2003/ Returned for modification 22 April 2003/ Accepted 23 May 2003

Escherichia hermannii showed a low level of resistance to amoxicillin and ticarcillin, reversed by clavulanate, and a moderate susceptibility to piperacillin but was susceptible to all cephalosporins. A bla gene was cloned and encoded a typical class A ß-lactamase (HER-1, pI 7.5), which shares 45, 44, 41, and 40% amino acid identity with other ß-lactamases, AER-1 from Aeromonas hydrophila, MAL-1/Cko-1 from Citrobacter koseri, and TEM-1 and LEN-1, respectively. No ampR gene was detected. Only penicillins were efficiently hydrolyzed, and no hydrolysis was observed for cefuroxime and broad-spectrum cephalosporins. Sequencing of the bla gene in 12 other strains showed 98 to 100% identity with blaHER-1.

* Corresponding author. Mailing address: Service de Bactériologie, Hôpital Tenon, 4, rue de la Chine, 75670 PARIS Cedex 20, France. Phone: 33 1 56 01 70 18. Fax: 33 1 56 01 61 08. E-mail: guillaume.arlet{at}tnn.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, August 2003, p. 2669-2673, Vol. 47, No. 8
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.8.2669-2673.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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