This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wain, J. Articles by Dougan, G. Search for Related Content PubMed PubMed Citation Articles by Wain, J. Articles by Dougan, G.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, September 2003, p. 2732-2739, Vol. 47, No. 9
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.9.2732-2739.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Analysis of incHI1 Antimicrobial Resistance Plasmids from Salmonella Serovar Typhi Strains Associated with Typhoid Fever

John Wain,^1,2,3* L. T. Diem Nga,¹ Claire Kidgell,² Keith James,⁴ Sarah Fortune,^1, To Song Diep,⁵ Tahir Ali,^2, Peadar Ó Gaora,² Christopher Parry,^1,3 Julian Parkhill,⁴ Jeremy Farrar,^1,3 Nicholas J. White,^1,3 and Gordon Dougan²

Wellcome Trust Clinical Research Unit,¹ Centre for Tropical Diseases, Ho Chi Minh City, Vietnam,⁵ Centre for Molecular Microbiology and Infection, Department of Biological Sciences and Division of Investigative Sciences, Imperial College, London,² Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge,⁴ Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Oxford University, Oxford, United Kingdom³

Received 16 December 2002/ Returned for modification 4 May 2003/ Accepted 4 June 2003

The first outbreak of multidrug-resistant (MDR) typhoid fever in Vietnam was in 1993, and by 1995 nearly 90% of cases were MDR. Plasmid HCM1, sequenced in full, is an incHI1 plasmid from Salmonella enterica serovar Typhi strain CT18, isolated in Vietnam in 1993. Restriction analysis shows that pHCM1 shares a restriction fragment length polymorphism (RFLP) pattern with plasmids isolated from the first outbreak and 10 of 17 MDR plasmids isolated from sporadic cases occurring at the same time in Vietnam. A core region of pHCM1 has significant DNA sequence similarity to plasmid R27, isolated in 1961 from S. enterica in the United Kingdom. There are five regions of DNA in pHCM1 which are not present in R27. Two of these are putative acquisition regions; the largest is 34.955 kbp in length and includes sequences of several antibiotic resistance genes and several insertion sequences. The borders of this region are defined by two identical IS10 left elements, associated with an inversion of DNA or with a truncated Tn10 element. The second, smaller region is 14.751 kbp and carries a trimethoprim resistance gene dfr14A cassette associated with a class 1 integrase. In 1993 to 1994, restriction analysis revealed some variations in the structures of Salmonella serovar Typhi MDR plasmids which were mapped to the two putative acquisition regions and three smaller variable regions. In 1996 a single RFLP type, RFLP7, was found to carry the dfrA7 and sul-1 genes, which were not present on R27 or pHCM1. This plasmid type appears to have a selective advantage over other plasmids with the same resistance phenotype.

Present address: Division of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Mass.

Present address: Academic Pediatrics, St. Mary's Medical School, Imperial College, London, United Kingdom.

This article has been cited by other articles:

• Chau, T. T., Campbell, J. I., Galindo, C. M., Van Minh Hoang, N., Diep, T. S., Nga, T. T. T., Van Vinh Chau, N., Tuan, P. Q., Page, A. L., Ochiai, R. L., Schultsz, C., Wain, J., Bhutta, Z. A., Parry, C. M., Bhattacharya, S. K., Dutta, S., Agtini, M., Dong, B., Honghui, Y., Anh, D. D., Canh, D. G., Naheed, A., Albert, M. J., Phetsouvanh, R., Newton, P. N., Basnyat, B., Arjyal, A., La, T. T. P., Rang, N. N., Phuong, L. T., Van Be Bay, P., von Seidlein, L., Dougan, G., Clemens, J. D., Vinh, H., Hien, T. T., Chinh, N. T., Acosta, C. J., Farrar, J., Dolecek, C. (2007). Antimicrobial Drug Resistance of Salmonella enterica Serovar Typhi in Asia and Molecular Mechanism of Reduced Susceptibility to the Fluoroquinolones. Antimicrob. Agents Chemother. 51: 4315-4323 [Abstract] [Full Text]  
• Choi, S. Y., Jeon, Y.-S., Lee, J. H., Choi, B., Moon, S. H., von Seidlein, L., Clemens, J. D., Dougan, G., Wain, J., Yu, J., Lee, J. C., Seol, S. Y., Lee, B. K., Song, J.-H., Song, M., Czerkinsky, C., Chun, J., Kim, D. W. (2007). Multilocus sequence typing analysis of Shigella flexneri isolates collected in Asian countries. J Med Microbiol 56: 1460-1466 [Abstract] [Full Text]  
• Nwaneshiudu, A. I., Mucci, T., Pickard, D. J., Okeke, I. N. (2007). A Second Large Plasmid Encodes Conjugative Transfer and Antimicrobial Resistance in O119:H2 and Some Typical O111 Enteropathogenic Escherichia coli Strains. J. Bacteriol. 189: 6074-6079 [Abstract] [Full Text]  
• WEILL, F.-X., TRAN, H. H., ROUMAGNAC, P., FABRE, L., MINH, N. B., STAVNES, T. L., LASSEN, J., BJUNE, G., GRIMONT, P. A.D., GUERIN, P. J. (2007). CLONAL RECONQUEST OF ANTIBIOTIC-SUSCEPTIBLE SALMONELLA ENTERICA SEROTYPE TYPHI IN SON LA PROVINCE, VIETNAM. Am J Trop Med Hyg 76: 1174-1181 [Abstract] [Full Text]  
• Holt, K. E., Thomson, N. R., Wain, J., Phan, M. D., Nair, S., Hasan, R., Bhutta, Z. A., Quail, M. A., Norbertczak, H., Walker, D., Dougan, G., Parkhill, J. (2007). Multidrug-Resistant Salmonella enterica Serovar Paratyphi A Harbors IncHI1 Plasmids Similar to Those Found in Serovar Typhi. J. Bacteriol. 189: 4257-4264 [Abstract] [Full Text]  
• Szczepanowski, R., Braun, S., Riedel, V., Schneiker, S., Krahn, I., Puhler, A., Schluter, A. (2005). The 120 592 bp IncF plasmid pRSB107 isolated from a sewage-treatment plant encodes nine different antibiotic-resistance determinants, two iron-acquisition systems and other putative virulence-associated functions. Microbiology 151: 1095-1111 [Abstract] [Full Text]  
• Kariuki, S., Revathi, G., Muyodi, J., Mwituria, J., Munyalo, A., Mirza, S., Hart, C. A. (2004). Characterization of Multidrug-Resistant Typhoid Outbreaks in Kenya. J. Clin. Microbiol. 42: 1477-1482 [Abstract] [Full Text]