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Antimicrobial Agents and Chemotherapy, September 2003, p. 2859-2863, Vol. 47, No. 9
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.9.2859-2863.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular and Biochemical Characterization of OXA-45, an Extended-Spectrum Class 2d' ß-Lactamase in Pseudomonas aeruginosa

Mark A. Toleman,1* Kenneth Rolston,2 Ronald N. Jones,3,4 and Timothy R. Walsh1

Department of Pathology and Microbiology, University of Bristol, Bristol BS8 1TD, United Kingdom,1 Anderson Medical Center, Houston, Texas,2 The JONES Group/JMI Laboratories, North Liberty, Iowa,3 the Tufts University School of Medicine, Boston, Massachusetts4

Received 3 January 2003/ Returned for modification 18 March 2003/ Accepted 20 June 2003

As part of the CANCER Antimicrobial Surveillance Program in North America, a clinical strain of Pseudomonas aeruginosa, strain 07-406, isolated in Texas was found to be resistant to all antimicrobials except polymyxin B. Genetic analysis of this isolate identified two unique extended-spectrum ß-lactamase genes. One, blaVIM-7, encoded a metallo-ß-lactamase (unpublished data), and the other, blaOXA-45, described here, encoded a class D extended-spectrum ß-lactamase. blaOXA-45 was isolated on a Sau3A1 genomic fragment of 1.8 kb and encodes a protein of 264 amino acids with the highest identities to OXA-18 (65.9%), OXA-9 (42.8%), OXA-22 (40.2%), OXA-12 (38.6%), and OXA-29 (35.2%) but weak identities with other class D ß-lactamases. blaOXA-45 was found to be harbored on a 24-kb plasmid in a region that displays high identities with a section of the 43-kb genomic island of Salmonella enterica serovar Typhimurium DT104. Biochemically OXA-45 is most similar to OXA-18 in its substrate profile and inhibition by clavulanic acid and is a member of the 2d' class of ß-lactamases.

* Corresponding author. Mailing address: Department of Pathology and Microbiology, University of Bristol, Bristol BS8 1TD, United Kingdom. Phone: 44 117 9287522. Fax: 44 117 9287896. E-mail: Mark.Toleman{at}bristol.ac.uk.

Antimicrobial Agents and Chemotherapy, September 2003, p. 2859-2863, Vol. 47, No. 9
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.9.2859-2863.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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