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Antimicrobial Agents and Chemotherapy, September 2003, p. 2864-2867, Vol. 47, No. 9
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.9.2864-2867.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Extended-Spectrum ß-Lactamases in Enterobacteriaceae in Buenos Aires, Argentina, Public Hospitals

M. Quinteros,1 M. Radice,2 N. Gardella,2 M. M. Rodriguez,2 N. Costa,1 D. Korbenfeld,1 E. Couto,1 G. Gutkind,2* and the Microbiology Study Group{dagger}

Hospital de Enfermedades Infecciosas "F. J. Muñiz",1 Facultad de Bioquímica y Farmacia UBA, Buenos Aires, Argentina2

Received 20 December 2002/ Returned for modification 21 March 2003/ Accepted 21 June 2003

Resistance to extended-spectrum cephalosporins is often associated with plasmid encoded extended spectrum ß-lactamases (ESBL). In order to evaluate the prevalence and diversity of ESBLs in enterobacteria in our city, a 1-month-period survey was carried out from April to May 2000. Extended-spectrum-cephalosporin-resistant strains, isolated from inpatient clinical specimens other than stools, were collected among 17 participating hospitals. From a total of 427 enterobacterial strains that were collected during this period, 39 were extended-spectrum cephalosporin resistant. The National Committee for Clinical Laboratory Standards' Screening and Confirmatory Tests for ESBL production were performed using cefotaxime and ceftazidime; cefepime and cefepime-clavulanic acid-containing disks were included. ß-Lactamases were characterized by isoelectric focusing and PCR amplification using specific primers. Three different ESBLs were detected: SHV-related (4 isolates), PER-2-type (9 isolates), and CTX-M-2-related (26 isolates). Sequencing of the corresponding genes confirmed CTX-M-2 in 19 of 21 and CTX-M-31 (an allelic variant) in the remaining 2 of 21. CTX-M-2 (or its variant) was detected in all Escherichia coli, Enterobacter aerogenes, Serratia marcescens, Proteus mirabilis, and Providencia stuartii strains, while PER-2 was detected in Enterobacter cloacae, E. aerogenes, and Klebsiella pneumoniae; SHV-related ESBL were found only in K. pneumoniae. These results clearly show that CTX-M-2 is the most prevalent ESBL produced by enterobacterial species isolated from public hospitals in Buenos Aires.

* Corresponding author. Mailing address: Hospital de Enfermedades Infecciosas "F. J. Muñiz," Buenos Aires, Argentina Facultad de Bioquímica y Farmacia UBA, Junín 956, CP:1113, Buenos Aires, Argentina. Phone: 54 11 496 48285. Fax: 54 11 450 83645. E-mail: ggutkind{at}ffyb.uba.ar.

{dagger} Members are listed in Acknowledgments.

Antimicrobial Agents and Chemotherapy, September 2003, p. 2864-2867, Vol. 47, No. 9
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.9.2864-2867.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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