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Antimicrobial Agents and Chemotherapy, September 2003, p. 2981-2983, Vol. 47, No. 9
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.9.2981-2983.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

A New TEM-Derived Extended-Spectrum ß-Lactamase (TEM-91) with an R164C Substitution at the {Omega}-Loop Confers Ceftazidime Resistance

Hiroshi Kurokawa, Naohiro Shibata, Yohei Doi, Keigo Shibayama, Kazunari Kamachi, Tetsuya Yagi, and Yoshichika Arakawa*

Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan

Received 12 February 2003/ Returned for modification 28 April 2003/ Accepted 4 June 2003

A new plasmid-mediated TEM-derived extended-spectrum ß-lactamase, TEM-91, was identified in a ceftazidime-resistant (MIC, >128 µg per ml) Escherichia coli strain isolated in 1996 in Japan. TEM-91 has three amino acid substitutions, R164C, M184T, and E240K, compared with TEM-1 penicillinase. The isoelectric point (pI), Km, and kcat of TEM-91 for ceftazidime were 5.7, 179 µM, and 29.0 s-1, respectively. The Ki of clavulanic acid for ceftazidime hydrolysis was 30.3 nM.

* Corresponding author. Mailing address: Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4-7-1 Musashi-Murayama, Tokyo 208-0011, Japan. Phone: 81 42 561 0771, ext. 500. Fax: 81 42 561 7173. E-mail: yarakawa{at}nih.go.jp.

Antimicrobial Agents and Chemotherapy, September 2003, p. 2981-2983, Vol. 47, No. 9
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.9.2981-2983.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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