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Antimicrobial Agents and Chemotherapy, September 2003, p. 3018-3020, Vol. 47, No. 9
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.9.3018-3020.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Disk Diffusion-Based Methods for Determining Candida parapsilosis Susceptibility to Anidulafungin

Zekaver Odabasi,1* Victor Paetznick,1 Beth P. Goldstein,2 John H. Rex,1,{dagger} and Luis Ostrosky-Zeichner1

Laboratory of Mycology Research, Division of Infectious Diseases, University of Texas—Houston Medical School, Houston, Texas 77030,1 Vicuron Pharmaceuticals, Inc., King of Prussia, Pennsylvania 194062

Received 25 April 2003/ Returned for modification 23 May 2003/ Accepted 11 June 2003

Zone diameters for anidulafungin by disk diffusion for 139 isolates of C. parapsilosis were compared with MICs by NCCLS M27-A2 broth microdilution. The comparison was poor unless the disks were prepared by dissolving anidulafungin in 1% dimethyl sulfoxide plus 0.1% Tween 80 and testing on Mueller-Hinton agar flooded with glucose and methylene blue.

* Corresponding author. Mailing address: Laboratory of Mycology Research, Division of Infectious Diseases, University of Texas—Houston Medical School, 6431 Fannin, JFB 1.728, Houston, TX 77030. Phone: (713) 500-5383. Fax: (713) 500-5495. E-mail: zekaver.odabasi{at}uth.tmc.edu.

{dagger} Present address: AstraZeneca Pharmaceuticals, Macclesfield, United Kingdom SK10 4TF.

Antimicrobial Agents and Chemotherapy, September 2003, p. 3018-3020, Vol. 47, No. 9
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.9.3018-3020.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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