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Antimicrobial Agents and Chemotherapy, September 2003, p. 3021-3024, Vol. 47, No. 9
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.9.3021-3024.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Real-Time PCR as a Versatile Tool for Investigating the Susceptibility of Human Herpesvirus 6 to Antiviral Agents

Muriel Macé,¹ Chaysavanh Manichanh,^1, Pascale Bonnafous,¹ Stéphanie Précigout,¹ David Boutolleau,¹ Agnès Gautheret-Dejean,^1,2 and Henri Agut^1*

Laboratoire de Virologie, UPRES EA2387, CERVI, Groupe Hospitalier Pitié-Salpêtrière, 75013 Paris,¹ Laboratoire de Microbiologie, Faculté des Sciences Pharmaceutiques et Biologiques, 75006 Paris, France²

Received 27 December 2002/ Returned for modification 10 April 2003/ Accepted 24 June 2003

A quantitative real-time PCR assay was developed for the determination of antiviral drug susceptibility and growth kinetics of human herpesvirus 6. The susceptibility and fitness of a sensitive strain, HST, and its ganciclovir-resistant derivative, GCVR1, were then characterized, leading us to conclude that the mutations of this latter virus did not alter its fitness significantly.

Present address: UEPSD-INRA, Domaine de Vilbert, 78352 Jouy-en-Josas Cedex, France.

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