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Antimicrobial Agents and Chemotherapy, January 2004, p. 275-280, Vol. 48, No. 1
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.1.275-280.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Vancomycin-Resistant Staphylococcus aureus Isolate from a Patient in Pennsylvania

Fred C. Tenover,1* Linda M. Weigel,1 Peter C. Appelbaum,2 Linda K. McDougal,1 Jasmine Chaitram,1 Sigrid McAllister,1 Nancye Clark,1 George Killgore,1 Caroline M. O'Hara,1 Laura Jevitt,1 Jean B. Patel,1 and Bülent Bozdogan2

Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333,1 Department of Pathology, Hershey Medical Center, Hershey, Pennsylvania 170332

Received 5 August 2003/ Returned for modification 25 September 2003/ Accepted 2 October 2003

A vancomycin-resistant Staphylococcus aureus (VRSA) isolate was obtained from a patient in Pennsylvania in September 2002. Species identification was confirmed by standard biochemical tests and analysis of 16S ribosomal DNA, gyrA, and gyrB sequences; all of the results were consistent with the S. aureus identification. The MICs of a variety of antimicrobial agents were determined by broth microdilution and macrodilution methods following National Committee for Clinical Laboratory Standards (NCCLS) guidelines. The isolate was resistant to vancomycin (MIC = 32 µg/ml), aminoglycosides, ß-lactams, fluoroquinolones, macrolides, and tetracycline, but it was susceptible to linezolid, minocycline, quinupristin-dalfopristin, rifampin, teicoplanin, and trimethoprim-sulfamethoxazole. The isolate, which was originally detected by using disk diffusion and a vancomycin agar screen plate, was vancomycin susceptible by automated susceptibility testing methods. Pulsed-field gel electrophoresis (PFGE) of SmaI-digested genomic DNA indicated that the isolate belonged to the USA100 lineage (also known as the New York/Japan clone), the most common staphylococcal PFGE type found in hospitals in the United States. The VRSA isolate contained two plasmids of 120 and 4 kb and was positive for mecA and vanA by PCR amplification. The vanA sequence was identical to the vanA sequence present in Tn1546. A DNA probe for vanA hybridized to the 120-kb plasmid. This is the second VRSA isolate reported in the United States.

* Corresponding author. Mailing address: Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention (G08), 1600 Clifton Rd., Atlanta, GA 30333. Phone: (404) 639-3375. Fax: (404) 639-1381. E-mail: fnt1{at}cdc.gov.

Antimicrobial Agents and Chemotherapy, January 2004, p. 275-280, Vol. 48, No. 1
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.1.275-280.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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